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首页> 外文期刊>Aquaculture >Evaluation of graded levels of soy oil as a fish oil replacement in high soy protein feeds for juvenile red drum, Sciaenops ocellatus
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Evaluation of graded levels of soy oil as a fish oil replacement in high soy protein feeds for juvenile red drum, Sciaenops ocellatus

机译：大豆蛋白饲料中少量大豆蛋白饲料饲料分级水平的评分水平评价，Sciaenops Ocellatus

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A 9-week feeding trial was conducted with juvenile red drum, Sciaenops ocellatus, to evaluate the use of soy oil as a fish oil replacement. Three primary protein sources (fishmeal FM, soybean meal SBM, and soy protein concentrate SPC) were utilized with 100% fish oil (FM, SBM, SPC), 75% fish oil (SBM, SPC), or 50% fish oil (FM, SBM, SPC) as the lipid source. Traditional growth and performance metrics (specific growth rate, feed consumption, feed conversion ratio) were tracked and tissue samples (liver, muscle, plasma, adipose, and brain) were collected for gas chromatography-based fatty acid profiling. Ten lipid metabolism related genes were analyzed for potential expression differences between dietary treatments in liver and muscle tissues and whole body and fillet tissues were sampled for proximate composition analyses. Fortyfour fatty acids were measured by gas chromatography-flame ionization detector (GC-FID) and evaluated with principle component analysis and ANOVA to understand the dietary influence on lipid metabolism and health. Significant differences in growth rate were observed with the SBM 50% fish oil diet outperforming the FM 100% fish oil reference diet. All other soy protein-based diets performed statistically equivalent to both FM reference diets (100% and 50% fish oil) in regard to growth, however all soy protein-based formulations had significantly lower feed conversion ratios than the fishmeal-based references (p < .001). Gene expression differences were not significant in most cases, however often trended similarly as the observed performance. Fatty acid profiles differed as a function of oil source, with no apparent influence by protein source, with C18:2n-6 (linoleic acid) being-the primary differentiator. Overall, the six soy protein, fishmeal-free formulations performed equivalently or better than the fishmeal references with up to 50% of fish oil replaced by soybean oil.
机译：用少年红鼓，Sciaenops Oceletatus进行9周的饲养试验，以评估豆油作为鱼油替代品的使用。三种主要蛋白质来源（鱼粉FM，大豆膳食SBM和大豆蛋白浓缩物SPC）用于100％鱼油（FM，SBM，SPC），75％鱼油（SBM，SPC）或50％鱼油（FM ，SBM，SPC）作为脂质源。传统的生长和性能度量（特定的生长速率，饲料消耗，进料转化率）被跟踪，并收集组织样品（肝脏，肌肉，血浆，脂肪和脑），用于基于气相色谱的脂肪酸分析。分析了十种脂质代谢相关基因进行肝脏和肌肉组织中膳食治疗之间的潜在表达差异，并对全身和圆角组织进行采样以进行近似成分分析。通过气相色谱 - 火焰离子化检测器（GC-FID）测量Fortyfour脂肪酸，并用原理分析和Anova评估，以了解对脂质代谢和健康的膳食影响。随着SBM 50％鱼油饮食，观察到增长率的显着差异，优于FM 100％鱼油参考饮食。所有其他大豆蛋白的饮食在统计上相当于增长的FM参考饮食（100％和50％的鱼油），然而所有大豆蛋白的制剂比鱼拍的参考数显着降低了饲料转化比率（P. <.001）。在大多数情况下，基因表达差异并不显着，但通常会随着观察到的性能而与之相似。脂肪酸谱不同于油源的函数，蛋白质来源没有明显影响，C18：2N-6（亚油酸）是 - 主要分化剂。总体而言，六个大豆蛋白，无菌制剂等效或优于鱼粉参考，其含有高达50％的鱼油替代的豆油。

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