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首页> 外文期刊>Biotechnology Progress >Clearance of porcine circovirus and porcine parvovirus from porcine-derived pepsin by low pH inactivation and cation exchange chromatography
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Clearance of porcine circovirus and porcine parvovirus from porcine-derived pepsin by low pH inactivation and cation exchange chromatography

机译：通过低pH灭活和阳离子交换色谱法从猪衍生的胃蛋白和阳离子交换色谱间隙清关

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摘要

The contamination of oral rotavirus vaccines by porcine circovirus (PCV) raised questions about potential PCV contamination of other biological products when porcine trypsin or pepsin is used in production process. Several methods can be potentially implemented as a safety barrier when animal derived trypsin or pepsin is used. Removal of PCV is difficult by the commonly used viral filters with the pore size cutoff of approximately 20 nm because of the smaller size of PCV particles that are around 17 nm. It was speculated that operating the chromatography step at a pH higher than pepsin's low pI, but lower than pIs, of most viruses would allow the pepsin to flow through the resin and be recovered from the flow through pool whilst the viruses would be retained on the resin. In this study, we investigated low pH inactivation of viruses including PCV Type 1 (PCV1) and PCV1 removal by cation exchange chromatography (CEX) in the presence of pepsin. Both parvovirus and PCV1 could be effectively inactivated by low pH and PCV1 could be removed by POROS 50HS CEX. The POROS 50HS method presented in this article is helpful for designing other CEX methods for the same purpose and not much difference would be expected for similar product intermediates and same process parameters. While the effectiveness needs to be confirmed for specific applications, the results demonstrate that both low pH (pH 1.7) and CEX methods were successful in eliminating PCV1 and thus either can be considered as an effective virus barrier.
机译：通过猪胃肠病毒（PCV）口腔旋转疫苗疫苗的污染提出了关于猪胰蛋白酶或胃蛋白酶在生产过程中的其他生物产品的潜在PCV污染的问题。当使用动物衍生的胰蛋白酶或胃蛋白酶时，几种方法可以作为安全屏障。通过孔径截止约为20nm的常用病毒过滤器，除去PCV是难以大约20nm的难以达到约20nm的困难的。被推测，在高于胃蛋白酶的低p1的pH下操作色谱步骤，但低于PI，大多数病毒将使胃蛋白酶流过树脂并从流动池中回收，同时将病毒保留在池中树脂。在这项研究中，我们研究了在胃蛋白酶的存在下通过阳离子交换色谱（CEX）的PCV型1（PCV1）和PCV1去除的病毒的低pH失活。 Provovirus和PCV1都可以通过低pH和PCV1通过Poros 50HS Cex除去PCV1。本文中提出的Poros 50HS方法有助于为同一目的设计其他CEX方法，并且对类似产品中间体和相同的工艺参数预期不太差异。虽然需要确认特定应用的有效性，但结果表明，低pH（pH 1.7）和CEX方法在消除PCV1方面是成功的，因此可以被认为是有效的病毒屏障。

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