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Development of PCR-based DNA marker for detection of white carrot contamination caused by Y2 locus

机译：基于PCR的DNA标志物检测Y2位点引起的白胡萝卜污染

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摘要

In carrot (Daucus carota L.), the taproot colors orange, yellow and white are determined mostly by the Y, Y2, and Or loci. One of the most severe issues in carrot seed production is contamination by wild white carrot. To evaluate the contamination ratio, easily detectable DNA markers for white carrot are desired. To develop PCR-based DNA markers for the Y2 locus, we have re-sequenced two orange-colored carrot cultivars at our company (Fujii Seed, Japan), as well as six white- and one light-orange-colored carrots that contaminated our seed products. Within the candidate region previously reported for the Y2 locus, only one DNA marker, Y2_7, clearly distinguished white carrots from orange ones in the re-sequenced samples. The Y2_7 marker was further examined in 12 of the most popular hybrid orange cultivars in Japan, as well as 'Nantes' and 'Chantenay Red Cored 2'. The Y2_7 marker showed that all of the orange cultivars examined had the orange allele except for 'Beta-441'. False white was detected in the orange-colored 'Beta-441'. The Y2_7 marker detected white root carrot contamination in an old open-pollinated Japanese cultivar, 'Nakamura Senkou Futo'. This marker would be a useful tool in a carrot seed quality control for some cultivars.
机译：在胡萝卜（Daucus Carota L.）中，Taproot颜色橙色，黄色和白色主要由Y，Y2和或基因座确定。胡萝卜种子生产中最严重的问题之一是野生白胡萝卜污染。为了评估污染率，需要易于检测的白色红萝卜的DNA标记。为了为Y2基因座开发基于PCR的DNA标记，我们已经在我们公司（富士籽，日本）以及六个白色和一个浅橙色的胡萝卜（富士籽）重新排序了两种污染种子产品。在先前向Y2基因座报道的候选区域内，只有一个DNA标记，Y2_7，从重新测序样品中的橙色中的白色胡萝卜清楚地分化。在日本最受欢迎的混合橙色品种中进一步研究了Y2_7标记，以及“南特”和“唐宁红色核心2”。 Y2_7标记表明，除了'beta-441'外，检查的所有橙色品种都有橙色等位基因。在橙色的'beta-441'中检测到假白色。 Y2_7标记检测到旧的开放式日本品种中的白根红萝卜污染，'Nakamura Senkou Futo'。这个标记将是某些品种的胡萝卜种子质量控制中的有用工具。

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