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首页> 外文期刊>Analytical chemistry >Depth profiling of peptide films with TOF-SIMS and a C-60 probe
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Depth profiling of peptide films with TOF-SIMS and a C-60 probe

机译：使用TOF-SIMS和C-60探针对肽膜进行深度分析

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A buckminsterfullerene ion source is employed to characterize peptide-doped trehalose thin films. The experiments are designed to utilize the unique sputtering properties of cluster ion beams for molecular depth profiling. The results show that trehalose films with high uniformity can be prepared on Si by a spin-coating technique. Bombardment of the film with C-60(+) results in high quality time-of-flight secondary ion mass spectrometry spectra, even during ion doses of up to 3 x 10(14) ions/cm(2). This result is in contrast to atomic bombardment experiments in which the dose of incident ions must be kept below 10(12) ions/cm(2) so as to retain mass spectral information. Moreover, since the films are of uniform thickness, it is possible to depth-profile through the film and into the Si substrate. This experimental protocol allows the yield of trehalose molecular equivalents and the degree of interface mixing to be evaluated in detail. When doped with a variety of small peptides up to a molecular weight of m/z 500, we find that the peptide molecular ion intensity remains stable under continuous C-60(+) bombardment, although some decrease in intensity is observed. The results are interpreted in terms of a model whereby the high trehalose yield and low damage depth of the C-60 projectile combine to prevent damage accumulation. In general, the peptide-trehalose system provides a valuable model for evaluating the parameters that lead to effective 3-dimensional characterization of biomaterials.
机译：使用巴克敏斯特富勒烯离子源来表征肽掺杂的海藻糖薄膜。设计这些实验是为了利用簇离子束的独特溅射特性进行分子深度分析。结果表明，可以通过旋涂技术在Si上制备高均匀度的海藻糖膜。用C-60（+）轰击薄膜可产生高质量的飞行时间二次离子质谱图，即使在离子剂量高达3 x 10（14）离子/ cm（2）的情况下也是如此。该结果与原子轰击实验相反，在原子轰击实验中，入射离子的剂量必须保持在10（12）离子/ cm（2）以下，以保留质谱信息。此外，由于膜具有均匀的厚度，因此可以对穿过膜并进入Si衬底的深度进行轮廓分析。该实验方案允许详细评估海藻糖分子当量的产率和界面混合的程度。当掺入分子量高达m / z 500的各种小肽时，我们发现在连续C-60（+）轰击下，肽分子离子强度保持稳定，尽管观察到强度有所降低。用模型解释结果，C-60弹丸的高海藻糖产量和低损伤深度相结合，可以防止损伤积累。通常，肽-海藻糖系统可提供有价值的模型，用于评估可对生物材料进行有效3维表征的参数。

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