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首页> 外文期刊>Cellular immunology >Usefulness of IL-21, IL-7, and IL-15 conditioned media for expansion of antigen-specific CD8+T cells from healthy donor-PBMCs suitable for immunotherapy
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Usefulness of IL-21, IL-7, and IL-15 conditioned media for expansion of antigen-specific CD8+T cells from healthy donor-PBMCs suitable for immunotherapy

机译：IL-21，IL-7和IL-15条件培养基用于扩增抗原特异性CD8 + T细胞的培养基，适用于适合免疫疗法的健康供体 - PBMC

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摘要

Clonal anergy and depletion of antigen-specific CD8+ T cells are characteristics of immunosuppressed patients such as cancer and post-transplant patients. This has promoted translational research on the adoptive transfer of T cells to restore the antigen-specific cellular immunity in these patients. In the present work, we compared the capability of PBMCs and two types of mature monocyte-derived DCs (moDCs) to prime and to expand ex-vivo antigen-specific CD8+ T cells using culture conditioned media supplemented with IL-7, IL-15, and IL-21. The data obtained suggest that protocols involving moDCs are as efficient as PBMCs-based cultures in expanding antigen-specific CD8+ T cell to ELA and CMV model epitopes. These three gamma common chain cytokines promote the expansion of naive-like and central memory CD8+ T cells in PBMCs-based cultures and the expansion of effector memory T cells when moDCs were used. Our results provide new insights into the use of media supplemented with IL-7, IL-15, and IL-21 for the in-vitro expansion of early-differentiated antigen-specific CD8+ T cells for immunotherapy purposes.y
机译：克隆无能和抗原特异性CD8+T细胞耗竭是免疫抑制患者（如癌症和移植后患者）的特征。这促进了T细胞过继转移的转化研究，以恢复这些患者的抗原特异性细胞免疫。在目前的工作中，我们比较了PBMC和两种成熟单核细胞衍生DC（moDCs）使用补充有IL-7、IL-15和IL-21的培养条件培养基启动和扩增体外抗原特异性CD8+T细胞的能力。获得的数据表明，在将抗原特异性CD8+T细胞扩增为ELA和CMV模型表位方面，涉及MODC的方案与基于PBMC的培养基一样有效。这三种γ-共链细胞因子促进基于PBMC的培养物中幼稚型和中央记忆CD8+T细胞的扩增，以及使用MODC时效应记忆T细胞的扩增。我们的结果为使用补充有IL-7、IL-15和IL-21的培养基体外扩增早期分化抗原特异性CD8+T细胞进行免疫治疗提供了新的见解。Y

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