• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Animal Science >Interactions of amino acids and hormones regulate the balance between growth and milk protein synthesis in lactating rats fed diets differing in protein content
【24h】

Interactions of amino acids and hormones regulate the balance between growth and milk protein synthesis in lactating rats fed diets differing in protein content

机译：氨基酸和激素的相互作用调节哺乳大鼠生长和牛奶蛋白合成之间的平衡，喂养蛋白质含量不同的饮食

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Insulin-like growth factor-I (IGF-I), growth hormone (GH), and prolactin (PRL) play important roles in milk protein synthesis, and their plasma concentrations were reported to be affected by dietary protein intake. To investigate the relationship between circulating amino acid (AA) and concentrations of these hormones, 18 Wistar rats aged 14 wk were assigned to a low (LP; 9% protein), standard (SP; 21% protein), or high-protein (HP; 35% protein) diet from parturition through day 15 of lactation. Plasma, liver, pituitary gland, skeletal muscle, and mammary gland samples were collected at the end of treatment. Circulating and hepatic IGF-I concentrations increased linearly with elevated dietary protein concentrations (P < 0.0001). Rats receiving the HP diet had higher circulating GH (P < 0.01) and pituitary PRL concentrations (P < 0.0001) but lower pituitary GH concentration (P < 0.0001) relative to those in rats receiving the LP and SP diets. Pearson correlation test performed on composed data across treatments showed that several circulating AAs were correlated with circulating and tissue concentrations of IGF-I, GH, and PRL. Multiple linear regression analyses identified Leu, Gln, Ala, Gly, and Arg as the main AAs associated with hormone responses (R-2 = 0.37 similar to 0.80; P < 0.05). Rats fed the LP and HP diets had greater Igf1 and Ghr gene expression in skeletal muscle than those fed the SP diets (P < 0.01). However, LP treatment decreased Prlr mRNA abundance in mammary glands as compared with the SP and HP treatments (P < 0.05). The HP diets increased AA transporter expression (P < 0.01) but decreased mammalian target of rapamycin (P < 0.05) and 70 kDa ribosomal protein S6 kinase 1 (P < 0.01) phosphorylation in mammary glands as compared with the LP and SP diets. The results of the present study suggested that several circulating AAs mediated the effects of dietary protein supply on concentrations of IGF-I, GH, and PRL, which in turn altered the metabolism status in peripheral tissues including the lactating mammary glands.
机译：胰岛素样生长因子-I（IGF-I）、生长激素（GH）和催乳素（PRL）在牛奶蛋白质合成中起重要作用，据报道，它们的血浆浓度受膳食蛋白质摄入量的影响。为了研究循环氨基酸（AA）与这些激素浓度之间的关系，18只14周龄的Wistar大鼠从分娩到哺乳第15天被分配到低（LP；9%蛋白质）、标准（SP；21%蛋白质）或高蛋白（HP；35%蛋白质）饮食。在治疗结束时收集血浆、肝脏、垂体、骨骼肌和乳腺样本。循环和肝脏IGF-I浓度随膳食蛋白质浓度升高而线性增加（P<0.0001）。与接受LP和SP饮食的大鼠相比，接受HP饮食的大鼠的循环GH（P<0.01）和垂体PRL浓度较高（P<0.0001），但垂体GH浓度较低（P<0.0001）。对不同处理的合成数据进行的皮尔逊相关检验表明，几种循环AAs与IGF-I、GH和PRL的循环和组织浓度相关。多元线性回归分析表明，Leu、Gln、Ala、Gly和Arg是与激素反应相关的主要AAs（R-2=0.37，类似于0.80；P<0.05）。喂食LP和HP饮食的大鼠骨骼肌中Igf1和Ghr基因的表达高于喂食SP饮食的大鼠（P<0.01）。然而，与SP和HP处理相比，LP处理降低了乳腺中Prlr mRNA的丰度（P<0.05）。与LP和SP饮食相比，HP饮食增加了AA转运蛋白的表达（P<0.01），但降低了乳腺中雷帕霉素的哺乳动物靶点（P<0.05）和70 kDa核糖体蛋白S6激酶1（P<0.01）磷酸化。本研究结果表明，几种循环AAs介导了膳食蛋白质供应对IGF-I、GH和PRL浓度的影响，进而改变了包括泌乳乳腺在内的外周组织的代谢状态。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号