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首页> 外文期刊>Agronomia Costarricense >COMPARISON OF THREE in vitro PROTOCOLS FOR DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION OF Coffea arabica L. CVS. CATURRA AND CATUAI
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COMPARISON OF THREE in vitro PROTOCOLS FOR DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION OF Coffea arabica L. CVS. CATURRA AND CATUAI

机译：三种阿拉伯咖啡直接体细胞胚发生和植株再生的体外方案比较。卡图拉和卡图伊

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摘要

A coffee (Coffea arábica L. cvs. Caturra and Catuaí) plant regeneration protocol via direct somatic embryogenesis was established. Vitroplant explants from first and second pair of leaves and explants from the distal, middle, and basal sections of 3and 12 month-old plants were cultivated on the protocols described by Yasuda, Hatanaka and CATIE. Caturra somatic embryos were cultured on the same embryo induction media or DEV medium. The maximum number of somatic embryos (2.71±0.45) was obtained from Caturra vitroplants. Explants from 3 month-old plants showed better response than 12 month-old Caturra explants. No somatic embryos were obtained in the in vitro protocols evaluated with 12 month-old Catuaí rojo plants. No differences were observed onthe number of embryos produced from the first and second pair of vitroplant leaves and sections of the distal, middle, and basal coffee plant leaves. The Yasuda protocol was the most efficient to induce direct somatic embryogenesis and embryo-to-plant conversion for the Caturra variety, whereas Hatanaka's was the most suitable for the Catuaí variety.
机译：建立了通过直接体细胞胚发生的咖啡（CoffeaarábicaL. cvs。Caturra和Catuaí）植物再生方案。按照Yasuda，Hatanaka和CATIE所述的方法，种植来自第一对和第二对叶子的玻璃体外植体以及来自3个月和12个月大植物的远侧，中部和基础部分的外植体。 Caturra体细胞胚在相同的胚诱导培养基或DEV培养基上培养。从Caturra试管植物获得最大数量的体细胞胚（2.71±0.45）。 3个月大植物的外植体表现出比12个月大的Caturra外植体更好的响应。在12个月大的Catuaírojo植物评估的体外方案中，未获得体细胞胚。从第一对和第二对离体植物叶片以及远侧，中部和基层咖啡植物叶片的切片产生的胚数目没有观察到差异。 Yasuda协议最有效地诱导了Caturra品种的直接体细胞胚发生和胚胎到植物的转化，而Hatanaka协议最适合于Catuaí品种。

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