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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Liquid chromatography-tandem mass spectrometry of porphyrins and porphyrinogens in biological materials: Separation and identification of interfering poly(ethylene) glycol by travelling wave ion mobility spectrometry/tandem mass spectrometry
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Liquid chromatography-tandem mass spectrometry of porphyrins and porphyrinogens in biological materials: Separation and identification of interfering poly(ethylene) glycol by travelling wave ion mobility spectrometry/tandem mass spectrometry

机译：生物材料中卟啉和卟啉原的液相色谱-串联质谱法：行波离子迁移率/串联质谱法分离和鉴定干扰性聚乙二醇

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摘要

Biological and clinical samples for porphyrin and porphyrinogen analyses by liquid chromatography-tandem mass spectrometry (LC-MS/MS) are often contaminated with poly(ethylene)glycol (PEG), which complicates the interpretation of mass spectra and characterisation of new porphyrin metabolites. Two contaminating PEG molecules (m/z 833 and m/z 835) were completely separated from uroporphyrin I (m/z 831) by travelling wave ion mobility spectrometry and characterised by tandem mass spectrometry. One of the PEG species (m/z 835) also co-eluted with uroporphyrinogen I (m/z 837) and was unresolvable by travelling wave ion mobility spectrometry/MS, therefore contaminating the MS/MS mass spectra owing to isotope distribution. These PEG species, with the [M + H]+ ions at m/z at 833 and/or m/z 835, co-eluted with uroporphyrin I and uroporphyrinogen I by LC-MS/MS and could be wrongly identified as uroporphomethenes.
机译：液相色谱-串联质谱法（LC-MS / MS）用于分析卟啉和卟啉原的生物学和临床样品经常被聚（乙二醇）乙二醇（PEG）污染，这使质谱的解释和新卟啉代谢物的表征变得复杂。通过行波离子迁移率光谱法将两个污染的PEG分子（m / z 833和m / z 835）与尿卟啉I（m / z 831）完全分离，并通过串联质谱法进行了表征。 PEG种类之一（m / z 835）也与尿卟啉原I（m / z 837）共洗脱，并且不能通过行波离子迁移率质谱/ MS解析，因此由于同位素分布而污染了MS / MS质谱图。这些PEG物种的[M + H] +离子在833和/或m / z 835的m / z处通过尿卟啉I和尿卟啉原I共同被LC-MS / MS洗脱，可能被错误地鉴定为尿卟啉。

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