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首页> 外文期刊>Analytical and bioanalytical chemistry >Multi-modal applicability of a reversed-phase/weak-anion exchange material in reversed-phase, anion-exchange, ion-exclusion, hydrophilic interaction and hydrophobic interaction chromatography modes
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Multi-modal applicability of a reversed-phase/weak-anion exchange material in reversed-phase, anion-exchange, ion-exclusion, hydrophilic interaction and hydrophobic interaction chromatography modes

机译：反相/弱阴离子交换材料在反相，阴离子交换，离子排斥，亲水相互作用和疏水相互作用色谱模式下的多峰适用性

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摘要

We recently introduced a mixed-mode reversed-phase/weak anion-exchange type separation material based on silica particles which consisted of a hydrophobic alkyl strand with polar embedded groups (thioether and amide functionalities) and a terminal weak anion-exchange-type quinuclidine moiety. This stationary phase was designed to separate molecules by lipophilicity and charge differences and was mainly devised for peptide separations with hydroorganic reversed-phase type elution conditions. Herein, we demonstrate the extraordinary flexibility of this RP/WAX phase, in particular for peptide separations, by illustrating its applicability in various chromatographic modes. The column packed with this material can, depending on the solute character and employed elution conditions, exploit attractive or repulsive electrostatic interactions, and/or hydrophobic or hydrophilic interactions as retention and selectivity increments. As a consequence, the column can be operated in a reversed-phase mode (neutral compounds), anion-exchange mode (acidic compounds), ion-exclusion chromatography mode (cationic solutes), hydrophilic interaction chromatography mode (polar compounds), and hydrophobic interaction chromatography mode (e.g., hydrophobic peptides). Mixed-modes of these chromatographic retention principles may be materialized as well. This allows an exceptionally flexible adjustment of retention and selectivity by tuning experimental conditions. The distinct separation mechanisms will be outlined by selected examples of peptide separations in the different modes.
机译：最近，我们推出了一种基于二氧化硅颗粒的混合模式反相/弱阴离子交换型分离材料，该材料由具有极性嵌入基团（硫醚和酰胺官能团）的疏水性烷基链和一个末端弱阴离子交换型奎宁环部分组成。该固定相旨在通过亲脂性和电荷差异来分离分子，主要设计用于在有机反相类型洗脱条件下进行肽分离。本文中，我们通过说明其在各种色谱模式下的适用性，证明了该RP / WAX相的非凡灵活性，特别是对于肽分离。填充这种材料的色谱柱可以根据溶质特性和采用的洗脱条件，利用吸引或排斥的静电相互作用和/或疏水或亲水的相互作用作为保留度和选择性的增量。因此，该色谱柱可以在反相模式（中性化合物），阴离子交换模式（酸性化合物），离子排阻色谱模式（阳离子溶质），亲水相互作用色谱模式（极性化合物）和疏水性模式下操作相互作用色谱模式（例如疏水肽）。这些色谱保留原理的混合模式也可以实现。通过调整实验条件，可以对保留和选择性进行非常灵活的调整。不同的分离机制将通过不同模式下肽段分离的选定实例进行概述。

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