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首页> 外文期刊>Analytical and bioanalytical chemistry >Determination of fortified and endogenous folates in food-based Standard Reference Materials by liquid chromatography-tandem mass spectrometry
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Determination of fortified and endogenous folates in food-based Standard Reference Materials by liquid chromatography-tandem mass spectrometry

机译：液相色谱-串联质谱法测定食品基标准参考物质中的强化和内源性叶酸

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摘要

The National Institute of Standards and Technology(NIST) is developing a wide variety of Standard Reference Materials (SRMs) to support measurements of vitamins and other nutrients in foods. Previously, NIST has provided SRMs with values assigned for the folate vitamer, folic acid (pteroylglutamic acid), which is fortified in several foods due to its role in prevention of neural tube defects. In order to expand the number of food-based SRMs with values assigned for folic acid, as well as additional endogenous folates, NIST has developed methods that include trienzyme digestion and isotope-dilution liquid chromatography-tandem mass spectrometric(LC-MS/MS) analysis. Sample preparation was optimized for each individual food type, but all samples were analyzed under the same LC-MS/MS conditions. The application of these methods resulted in folic acid values for SRM 1849a Infant/Adult Nutritional Formula and SRM 3233 Fortified Breakfast Cereal of (2.33±0.06) μg/g and (16.0±0.7) μg/g, respectively. In addition, the endogenous folate vitamer 5-methlytetrahydrofolate (5-MTHF) was detected and quantified in SRM 1849a Infant/Adult Nutritional Formula, candidate SRM 1549a Whole Milk Powder, and candidate SRM 1845a Whole Egg Powder, resulting in values of (0.0839±0.0071) μg/g, (0.211±0.014) μg/g, and (0.838±0.044) μg/g, respectively. SRM 1849a Infant/Adult Nutritional Formula is the first food-based NIST SRM to possess a reference value for 5-MTHF and the first certified reference material to have an assigned 5-MTHF value based on LC-MS/MS. The values obtained for folic acid and 5-MTHF by LC-MS/MS will be incorporated into the final value assignments for all these food-based SRMs.
机译：美国国家标准技术研究院（NIST）正在开发各种标准参考材料（SRM），以支持食品中维生素和其他营养物质的测量。以前，NIST为SRM提供了叶酸维生素，叶酸（蝶酰谷氨酸）的赋值，由于其在预防神经管缺陷中的作用，因此在多种食品中都得到了强化。为了增加为叶酸和其他内源性叶酸分配值的食品类SRM的数量，NIST已开发出包括三酶消化和同位素稀释液相色谱-串联质谱（LC-MS / MS）的方法分析。针对每种食品类型优化了样品制备，但所有样品均在相同的LC-MS / MS条件下进行分析。这些方法的应用导致SRM 1849a婴儿/成人营养配方奶粉和SRM 3233强化早餐谷物的叶酸值分别为（2.33±0.06）μg/ g和（16.0±0.7）μg/ g。此外，在SRM 1849a婴儿/成人营养配方奶粉，候选SRM 1549a全脂奶粉和候选SRM 1845a全蛋粉中检测并定量了内源性叶酸维他命5-甲基四氢叶酸（5-MTHF），得出的值为（0.0839±分别为0.0071）微克/克，（0.211±0.014）微克/克和（0.838±0.044）微克/克。 SRM 1849a婴儿/成人营养配方食品是第一个基于食品的NIST SRM，具有5-MTHF的参考值，并且是第一个经过认证的参考材料，具有基于LC-MS / MS的5-MTHF值。通过LC-MS / MS获得的叶酸和5-MTHF的值将纳入所有这些基于食物的SRM的最终值分配中。

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