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首页> 外文期刊>Analytical and bioanalytical chemistry >Determination of norfloxacin in human urine by capillary electrophoresis with electrochemiluminescence detection
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Determination of norfloxacin in human urine by capillary electrophoresis with electrochemiluminescence detection

机译：毛细管电泳-电化学发光法测定人尿中的诺氟沙星

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摘要

A fast and sensitive approach that can be used to detect norfloxacin in human urine using capillary electrophoresis with end-column electrochemiluminescence (ECL) detection of Ru(bpy)(3)(2+) is described. The separation column was a 75-mu m i. d. capillary. The running buffer was 15 mmol L-1 sodium phosphate (pH 8.2). The solution in the detection cell was 50 mmol L-1 sodium phosphate (pH 8.0) and 5 mmol L-1 Ru (bpy)(3)(2+). The ECL intensity varied linearly with norfloxacin concentration from 0.05 to 10 mu mol L-1. The detection limit (S/N= 3) was 0.0048 mu mol L-1, and the relative standard deviations of the ECL intensity and the migration time for eleven consecutive injections of 1.0 mu mol L-1 norfloxacin (n = 11) were 2.6% and 0.8%, respectively. The method was successfully applied to the determination of norfloxacin spiked in human urine without sample pretreatment. The recoveries were 92.7 -97.9%.
机译：描述了一种快速灵敏的方法，该方法可用于通过毛细管电泳和Ru（bpy）（3）（2+）的末端柱电化学发光（ECL）检测来检测人尿中的诺氟沙星。分离柱为75μmi。 d。毛细管。运行缓冲液是15 mmol L-1磷酸钠（pH 8.2）。检测池中的溶液是50 mmol L-1磷酸钠（pH 8.0）和5 mmol L-1 Ru（bpy）（3）（2+）。 ECL强度随诺氟沙星浓度从0.05到10μmolL-1线性变化。检出限（S / N = 3）为0.0048μmol L-1，连续十次注射1.0μmol L-1诺氟沙星（n = 11）的ECL强度和迁移时间的相对标准偏差为2.6分别为％和0.8％。该方法无需样品前处理即可成功地用于测定人尿中加氟诺沙星的含量。回收率为92.7 -97.9％。

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