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首页> 外文期刊>Annals of Applied Biology >Factors affecting the detection of potato mop-top virus in potato tubers and improvement of test procedures for more reliable assays
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Factors affecting the detection of potato mop-top virus in potato tubers and improvement of test procedures for more reliable assays

机译：影响马铃薯块茎中马铃薯拖把病毒检测的因素以及改进测试程序以进行更可靠的分析

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Progeny tubers were harvested from plants of 5 potato cultivars that had been grown in potato mop-top furovirus (PMTV) infested soil. The tubers were tested for virus infection by methods based on reverse transcriptase-polymerase chain reaction (RT-PCR) or ELISA incorporating monoclonal antibodies. Both RT-PCR and ELISA detected PMTV in extracts from tuber flesh. However, when the assay results were compared for individual tubers, there was an incomplete correlation between the 2 methods, and moreinfected tubers were detected by ELISA than by RT-PCR. PMTV was detected in some samples but not in others taken from different parts of the same tuber, and this uneven distribution may explain the discrepancy between the results of the ELISA and RT-PCRtests. Storage of tubers of 2 cultivars at an elevated temperature (20deg C) for 4 weeks prior to testing, nearly doubled the number of sample sites at which PMTV was detected by ELISA. Such pre-treatment may improve the accuracy of tuber tests. Most tubersof cultivars Saturna and Pentland Crown were symptomlessly infected suggesting that PMTV may easily escape detection in tubers of these cultivars if tests are based solely on presence of visual symptoms. The application of postharvest tuber testing, particularly to cultivars that do not display obvious symptoms, and the subsequent elimination of contaminated tubers will help to provide effective control of spread, and the establishment of PMTV at new sites.
机译：从5个马铃薯栽培品种的植物中收获后代块茎，这些植物已在马铃薯拖把顶部呋喃病毒（PMTV）感染的土壤中生长。通过基于逆转录酶-聚合酶链反应（RT-PCR）或掺有单克隆抗体的ELISA的方法对块茎进行病毒感染测试。 RT-PCR和ELISA均检测到块茎肉提取物中的PMTV。但是，当比较单个块茎的测定结果时，两种方法之间的关联不完全，并且通过ELISA检测到的块茎感染率要高于通过RT-PCR检测到的块茎。在从相同块茎不同部位采集的一些样品中检测到了PMTV，这种不均匀的分布可能解释了ELISA结果与RT-PCR测试之间的差异。在测试之前，将两个品种的块茎在升高的温度（20摄氏度）下存储4周，这几乎是通过ELISA检测PMTV的样品位点数量的两倍。这种预处理可以提高块茎测试的准确性。大多数Saturna和Pentland Crown的块茎均无症状感染，这表明如果仅根据视觉症状进行检测，PMTV可能很容易逃脱这些块茎的检测。采后块茎试验的应用，特别是对没有明显症状的栽培种的应用，随后消除受污染的块茎将有助于有效控制传播，并在新地点建立PMTV。

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