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Metagenomic assessment of body surface bacterial communities of the sea urchin, Tripneustes gratilla

机译：海胆体面细菌群体的偏见评估，Tripneustes Gratilla

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摘要

Sea urchins, including Tripneustes gratilla, are susceptible to a disease known as bald sea urchin disease, which has the potential to lead to economic losses in this emerging aquaculture industry in South Africa. This disease is characterized by lesions that form on sea urchin exoskeletal surfaces. This study aimed to characterize the body surface bacterial communities associated with T. gratilla, using a 16S rDNA gene metagenomics approach, to provide insight into the bacterial agents associated with this aquaculture species, as well as with this balding disease. Bacterial samples were collected from non-lesioned healthy animals obtained from natural locations along the eastern coast of South Africa, as well as from different cultured cohorts: non-lesioned healthy-, lesioned diseased- and non-lesioned stressed animals. A total of 1,067,515 individual bacterial operational taxonomic units (OTUs) were identified, belonging to 133 family-, 123 genus- and 113 species level OTU groups. Alpha diversity analyses, based on Chao1, Shannon and Simpson indices, showed that there were no statistically significant differences (ANOVA; P > 0.05) between the respective cohorts, as all cohorts displayed a high degree of bacterial diversity. Similarly, beta diversity analyses (Non-metric multidimensional scaling) showed a large degree of overlapping OTUs across the four cohorts. Within each cohort, various OTUs commonly associated with marine environments were found, predominantly belonging to the families Vibrionaceae, Saprospiraceae, Flavobacteriaceae and Sphingomonadaceae. Differential abundance analysis (DESeq2) revealed that OTUs that are differentially abundant across cohorts were likely not responsible for this balding disease, suggesting that complex bacterial agents, rather than a specific pathogenic agent, are likely causing this disease. Furthermore, the putative metabolic functions assigned to the bacterial communities showed that heterotrophic bacteria appear to be res
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  • 来源
    《Marine genomics》 |2019年第1期|共11页
  • 作者

    Brink M.; Rhode C.; Macey B. M.; Christison K. W.; Roodt-Wilding R.;

  • 作者单位

    Stellenbosch Univ Dept Genet Mol Breeding &

    Biodivers Grp Private Bag X1 ZA-7602 Stellenbosch Western Cape South Africa;

    Stellenbosch Univ Dept Genet Mol Breeding &

    Biodivers Grp Private Bag X1 ZA-7602 Stellenbosch Western Cape South Africa;

    Dept Agr Forestry &

    Fisheries Aquaculture Res Private Bag X2 ZA-8012 Cape Town Western Cape South Africa;

    Dept Agr Forestry &

    Fisheries Aquaculture Res Private Bag X2 ZA-8012 Cape Town Western Cape South Africa;

    Stellenbosch Univ Dept Genet Mol Breeding &

    Biodivers Grp Private Bag X1 ZA-7602 Stellenbosch Western Cape South Africa;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    16S rDNA; Bald sea urchin disease; Collector sea urchin; Next-generation sequencing; Opportunistic bacteria;

    机译：16s rdna;秃头海胆疾病;收藏家海胆;下一代测序;机会的细菌;

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