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首页> 外文期刊>Molecular ecology notes >Eleven new microsatellite markers for the tropical sea urchin Tripneustes gratilla and cross-amplification in Tripneustes ventricosa
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Eleven new microsatellite markers for the tropical sea urchin Tripneustes gratilla and cross-amplification in Tripneustes ventricosa

机译:热带海胆海豚草的11种新的微卫星标记和文氏海豚的交叉扩增

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摘要

We describe the isolation, characterization, and application of 11 polymorphic microsatellite loci from the tropical sea urchin Tripneustes gratilla. We genotyped a total of 73 individuals in three subpopulations (two in Hawaii, and one in the Philippines) and found between eight and 33 alleles per locus. We found significant heterozygote deficits within at least one subpopulation at seven out of 11 loci. We were successful at cross-amplifying four loci in the congeneric Tripneustes ventricosus. When used appropriately, this set of loci will be useful for quantifying population structure in Tripneustes urchins across broad geographical ranges.
机译:我们描述了11种多态微卫星基因座的分离,表征和从热带海胆tripneustes gratilla的应用。我们对3个亚群中的73个个体进行了基因分型(两个在夏威夷,一个在菲律宾),发现每个位点之间有8到33个等位基因。我们在11个基因座中的7个中的至少一个亚群中发现了显着的杂合子缺陷。我们成功地交叉扩增了同种的Tripneustes ventricosus中的四个基因座。如果使用得当,这组基因座将可用于在广阔的地理范围内定量海螯虾的种群结构。

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