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首页> 外文期刊>Animal Production Science >Changes in plasma oxidative stress biomarkers in dairy cows after oestrus synchronisation with controlled internal drug release (CIDR) and prostaglandinF(2 alpha) (PGF(2 alpha))
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Changes in plasma oxidative stress biomarkers in dairy cows after oestrus synchronisation with controlled internal drug release (CIDR) and prostaglandinF(2 alpha) (PGF(2 alpha))

机译:发情与受控内部药物释放(CIDR)和前列腺素F(2 alpha)(PGF(2 alpha))同步后,奶牛血浆氧化应激生物标志物的变化

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摘要

This study was designed to evaluate the plasma profiles of oxidative stress biomarkers, progesterone and ovarian follicle diameter in ovulatory versus an-ovulatory cows. Twenty cows were synchronised using controlled internal drug release (CIDR) and prostaglandinF(2 alpha) (PGF(2 alpha)) protocol. Plasma samples were analysed for progesterone (P4), oxidative stress (OS) biomarkers; reactive oxygen metabolites (ROMs), biological antioxidant potential (BAP), oxidative stress index (OSI = ROMs/BAP x 100), advanced oxidation protein products, ceruloplasmin and glutathione (GSH). Plasma P4 concentration was greater in ovulated cows 24 hours (h) after PGF(2 alpha) treatment but lower 48 h after PGF(2 alpha) treatment compared with that of an-ovulated cows at those sampling sessions (P < 0.05). Ovulated cows were diagnosed with greater ovarian follicle diameter compared with that of their herd mates not diagnosed for ovulation. Significant interaction of time of PGF(2 alpha) treatment and ovulation status (ovulatory versus an-ovulatory) with the plasma concentrations of OSI, BAP and GSH were observed. Ovulated cows had significantly lower BAP compared with that of an-ovulated cows (P < 0.05) 9 h, 48 h, 60 h and 128 h after PGF(2 alpha) treatment. Plasma concentrations of GSH were lower (P < 0.05) in ovulated cows than that of an-ovulated cows 60 h and 96 h after PGF(2 alpha) treatment. However, OSI was greater (P < 0.05) in ovulated cows than that of an-ovulated cows 9 h, 48 h, 60 h and 128 h after PGF(2 alpha) treatment. Significant associations were observed between OS status and sampling time. Oxidative stress status may have important physiological role in facilitating the ovulation process in oestrus synchronised dairy cows
机译:本研究旨在评估排卵性和排卵性母牛的氧化应激生物标志物,孕酮和卵巢卵泡直径的血浆分布。使用受控内部药物释放(CIDR)和前列腺素F(2 alpha)(PGF(2 alpha))方案同步处理20头母牛。分析血浆样品中的孕酮(P4),氧化应激(OS)生物标志物;活性氧代谢物(ROMs),生物抗氧化电位(BAP),氧化应激指数(OSI = ROMs / BAP x 100),高级氧化蛋白产物,铜蓝蛋白和谷胱甘肽(GSH)。 PGF(2 alpha)处理后排卵奶牛血浆P4浓度较高,而PGF(2 alpha)处理后48 h血浆血浆P4浓度较那些采样期的无排卵奶牛血浆P4浓度低(P <0.05)。与没有诊断排卵的牛群相比,被诊断为排卵的牛的卵泡直径更大。观察到PGF(2α)治疗时间和排卵状态(排卵与排卵)与OSI,BAP和GSH血浆浓度的显着相互作用。 PGF(2 alpha)处理后9h,48h,60h和128h,排卵奶牛的BAP显着低于排卵奶牛(P <0.05)。 PGF(2 alpha)处理后60h和96h,排卵奶牛血浆GSH浓度低于(P <0.05)。然而,在PGF(2 alpha)处理后9h,48h,60h和128h,排卵奶牛的OSI大于排卵奶牛的OSI(P <0.05)。在操作系统状态和采样时间之间观察到显着关联。氧化应激状态可能在促进发情期同步奶牛排卵过程中具有重要的生理作用

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