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首页> 外文期刊>Prostaglandins and Other Lipid Mediators >Postpartum levels of 8-iso-prostaglandin F-2 alpha in plasma and milk phospholipid fractions as biomarker of oxidative stress in first-lactating dairy cows
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Postpartum levels of 8-iso-prostaglandin F-2 alpha in plasma and milk phospholipid fractions as biomarker of oxidative stress in first-lactating dairy cows

机译：血浆和牛奶磷脂组分中的8-异前列腺素F-2α的产后水平是第一头泌乳奶牛氧化应激的生物标志

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F-2-isoprostanes such as 8-iso-prostaglandin F2 alpha (8-ISO-PGF(2 alpha)) are formed by free radical-catalyzed mechanisms from membrane phospholipids and from low density lipoproteins through peroxidation of arachidonic acid. Esterified 8-iso-PGF(2 alpha) is cleaved by phospholipases, circulates in blood and is excreted as putatively harmful oxidatively modified lipid via the kidney into urine. In this study we demonstrate that 8-iso-PGF(2 alpha) concentrations in plasma samples from heifers are higher (p < 0.005) compared to those from first-lactating dairy cows at 71 days postpartum. Furthermore, plasma 8-iso-PGF(2 alpha) concentrations vary with ovarian activity and differ in response to luteolytic initiation as well as activation of the hypothalamic pituitary gonadal axis between heifers and first-lactating cows. Sustainable concentrations of 8-iso-PGF(2 alpha) (50-150 pg/ml) are detectable in the phospholipid fraction of milk, suggesting milk as an additional excretion route for 8-isoprostanes. Plasma levels largely paralleled levels in milk (p < 0.001). Plasma phospholipid 8-iso-PGF2a concentrations in cyclic cows decreased (p < 0.05) from day 38 to day 71 postpartum, whereas milk phospholipid 8-iso-PGF(2 alpha) rather increased (p < 0.05). Cyclic cows tend to have higher 8-isoprostane levels compared to acyclic animals. In contrast to lipohydroperoxides, concentration of 8-iso-PGF(2 alpha) were not correlated with milk yield (p > 0.05). Our data indicate 8-iso-PGF(2 alpha) may be a novel biomarker of oxidative stress in dairy cow, which is detectable in blood as well as in milk. (C) 2014 Elsevier Inc. All rights reserved.
机译：F-2-异前列腺素如8-异前列腺素F2α（8-ISO-PGF（2 alpha））是通过自由基催化的机制从膜磷脂和低密度脂蛋白通过花生四烯酸的过氧化作用形成的。酯化的8-iso-PGF（2α）被磷脂酶切割，在血液中循环，并作为可能有害的氧化修饰脂质通过肾脏排泄到尿液中。在这项研究中，我们证明了在母牛产后71天，与首次泌乳奶牛的血浆样品相比，血浆样品中的8-iso-PGF（2 alpha）浓度更高（p <0.005）。此外，血浆8-异-PGF（2α）浓度随卵巢活动而变化，并且在对黄体和头胎母牛之间的黄体溶解垂体以及下丘脑垂体性腺轴的激活反应方面也不同。在牛奶的磷脂级分中可以检测到可持续浓度的8-iso-PGF（2 alpha）（50-150 pg / ml），这表明牛奶是8-异前列腺素的另一种排泄途径。血浆水平在很大程度上与牛奶中的水平平行（p <0.001）。从产后第38天到产后第71天，周期性奶牛的血浆磷脂8-iso-PGF2a浓度降低（p <0.05），而牛奶磷脂8-iso-PGF（2α）则升高（p <0.05）。与无环动物相比，环牛倾向于具有更高的8-异前列腺素水平。与脂质过氧化氢相比，8-iso-PGF（2α）的浓度与牛奶产量无关（p> 0.05）。我们的数据表明8-iso-PGF（2 alpha）可能是奶牛中氧化应激的一种新型生物标志物，在血液以及牛奶中均可检测到。 （C）2014 Elsevier Inc.保留所有权利。

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