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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Virus-induced gene silencing in Rauwolfia species
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Virus-induced gene silencing in Rauwolfia species

机译：RAUWOLFIA物种的病毒诱导基因沉默

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摘要

Elucidation of the monoterpene indole alkaloid biosynthesis has recently progressed in Apocynaceae through the concomitant development of transcriptomic analyses and reverse genetic approaches performed by virus-induced gene silencing (VIGS). While most of these tools have been primarily adapted for the Madagascar periwinkle (Catharanthus roseus), the VIGS procedure has scarcely been used on other Apocynaceae species. For instance, Rauwolfia sp. constitutes a unique source of specific and valuable monoterpene indole alkaloids such as the hypertensive reserpine but are also well recognized models for studying alkaloid metabolism, and as such would benefit from an efficient VIGS procedure. By taking advantage of a recent modification in the inoculation method of the Tobacco rattle virus vectors via particle bombardment, we demonstrated that the biolistic-mediated VIGS approach can be readily used to silence genes in both Rauwolfia tetraphylla and Rauwolfia serpentina. After establishing the bombardment conditions minimizing injuries to the transformed plantlets, gene downregulation efficiency was evaluated at approximately a 70% expression decrease in both species by silencing the phytoene desaturase encoding gene. Such a gene silencing approach will thus constitute a critical tool to identify and characterize genes involved in alkaloid biosynthesis in both of these prominent Rauwolfia species.
机译：近年来，随着转录组学分析和病毒诱导基因沉默（VIGS）的反向遗传方法的发展，在脱苦甘蓝科植物中对单萜吲哚生物碱生物合成的研究取得了进展。虽然这些工具中的大多数主要用于马达加斯加长春花（长春花），但VIGS程序很少用于其他夹竹桃科物种。例如，Rauwolfia sp.是一种独特的单萜吲哚生物碱来源，如高血压利血平，但也是研究生物碱代谢的公认模型，因此将受益于有效的VIGS程序。通过利用最近通过粒子轰击对烟草响尾蛇病毒载体的接种方法进行的修改，我们证明了基因敲除介导的VIGS方法可以很容易地用于沉默四叶萝芙木和蛇形萝芙木中的基因。在建立轰击条件以尽量减少对转化植株的伤害后，通过沉默植物烯去饱和酶编码基因，在两个物种中的表达降低约70%的情况下评估基因下调效率。因此，这种基因沉默方法将成为鉴定和表征这两种重要萝芙木属植物生物碱生物合成相关基因的关键工具。

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    Corbin Cyrielle; Lafontaine Florent; Sepulveda Liuda Johana; Carqueijeiro Ines; Courtois Martine; Lanoue Arnaud; de Bernonville Thomas Duge; Besseau Sebastien; Glevarec Gaelle; Papon Nicolas; Atehortua Lucia; Giglioli-Guivarch Nathalie; Clastre Marc; St-Pierre Benoit; Oudin Audrey; Courdavault Vincent;

  • 作者单位

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Angers Grp Etud Interact Hote Pathogene EA 3142 Angers France;

    Univ Antioquia Sede Invest Univ Lab Biotecnol Antioquia Medellin Colombia;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

    Univ Francois Rabelais Tours EA Biomol &

    Biotechnol Vegetales 2106 UFR Sci &

    Tech F-37200 Tours France;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞形态学;
  • 关键词

    Rauwolfia; Virus-induced gene silencing; Phytoene desaturase; Medicinal plant;

    机译：RAUWOLFIA;病毒诱导的基因沉默;植物去植物;药用植物;

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