...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Human and Experimental Toxicology >Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats
【24h】

Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats

机译：对氧化应激，肝化学代谢参数和大鼠疯狂摄入遗传毒性损伤的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A total of 66 male Wistar rats were used and six groups (control: 10 animals and experimental: 12 animals) were formed. While a separate control group was established for each study period, mad honey application to the animals in the experimental group was carried out with a single dose (12.5 g kg(-1) body weight (b.w.); acute stage), at a dose of 7.5 g kg(-1) b.w. for 21 days (subacute stage), and at a dose of 5 g kg(-1) b.w. for 60 days (chronic stage). Tissue and blood oxidative stress markers (malondialdehyde (MDA), nitric oxide (NO), 4-hydroxynonenal (HNE), superoxide dismutase, catalase, glutathione (GSH) peroxidase, and glucose-6-phosphate dehydrogenase), hepatic chemical metabolizing parameters in the liver (cytochrome P450 2E1, nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase, nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c reductase (CYTC), GSH S-transferase (GST), and GSH), and micronucleus and comet test in some samples were examined. Findings from the study showed that single and repeated doses given over the period increased MDA, NO, and HNE levels while decreasing/increasing tissue and blood antioxidant enzyme activities. From hepatic chemical metabolizing parameters, GST activity increased in the subacute and chronic stages and CYTC activity increased in the acute period, whereas GSH level decreased in the subacute stage. Changes in tail and head intensities were found in most of the comet results. Mad honey caused oxidative stresses for each exposure period and made some significant changes on the comet test in certain periods for some samples obtained. In other words, according to the available research results obtained, careless consumption of mad honey for different medical purposes is not appropriate.
机译：共使用66只雄性Wistar大鼠，形成六组（对照组：10只动物和实验组：12只动物）。虽然为每个研究期间设立了单独的对照组，但在实验组的动物中使用单剂量（12.5 g kg（-1）体重（体重）的蜂蜜；急性期），剂量为7.5克/千克（-1）体重，持续21天（亚急性期），剂量为5克/千克（-1）体重，持续60天（慢性期）。组织和血液氧化应激标记物（丙二醛（MDA）、一氧化氮（NO）、4-羟基壬醛（HNE）、超氧化物歧化酶、过氧化氢酶、谷胱甘肽（GSH）过氧化物酶和葡萄糖-6-磷酸脱氢酶）、肝脏化学代谢参数（细胞色素P450 2E1、烟酰胺腺嘌呤二核苷酸（NADH）-细胞色素b5还原酶、，检测了部分样本中的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-细胞色素c还原酶（CYTC）、GSH S-转移酶（GST）和GSH），以及微核和彗星试验。研究结果表明，在这段时间内，单次和重复给药会增加MDA、NO和HNE水平，同时降低/增加组织和血液中的抗氧化酶活性。从肝脏化学代谢参数来看，GST活性在亚急性期和慢性期增加，CYTC活性在急性期增加，而GSH水平在亚急性期降低。在大多数彗星结果中都发现了尾和头部强度的变化。疯狂蜂蜜在每个暴露期都会引起氧化应激，并在某些时期对获得的一些样本的彗星试验产生了一些显著变化。换句话说，根据获得的现有研究结果，出于不同的医疗目的不小心食用疯狂蜂蜜是不合适的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号