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首页> 外文期刊>Journal of Analytical Toxicology >Application of a Urine and Hair Validated LC-MS-MS Method to Determine the Effect of Hair Color on the Incorporation of 25B-NBOMe, 25C-NBOMe and 25I-NBOMe into Hair in the Rat
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Application of a Urine and Hair Validated LC-MS-MS Method to Determine the Effect of Hair Color on the Incorporation of 25B-NBOMe, 25C-NBOMe and 25I-NBOMe into Hair in the Rat

机译：尿液验证的LC-MS-MS方法的应用来确定毛发颜色对大鼠致氢毛发的掺入致氢的影响

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NBOMes are a group of new psychoactive substances derived from phenethylamines. Recreational abuse is thought to have begun in 2010 and they are commonly associated with the "club drug" scene. They are administered in liquid form or as blotters due to their high potency. An LC-MS-MS method was validated using Scientific Working Group for Forensic Toxicology parameters for the detection of 25B-, 25C- and 4-iodo-2,5-dimethoxy-N-[(2-methoxyphenyl) methyl]-benzeneethanamine (25I-NBOMe) using 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl) methyl]-benzeneethanamine (25B-NBOMe)-D-3 as internal standard for urine and hair. Calibration graphs with R-2 values > 0.99 were observed for urine and hair for concentrations ranging from 0.1 to 100 ng/mL and 0.025 to 2.5 ng/mg, respectively. Urine LODs ranged from 5 to 25 pg/mL and had an LOQ of 50 pg/mL. Hair LOD and LOQs ranged from 3 to 5 pg/mg and 6.25 to 12.5 pg/mg, respectively. Intra- and inter-day precision was <20% and accuracy was within +/- 20% for both matrices. The method was shown to be selective for both exogenous and endogenous compounds. No matrix effects were observed for either matrix. LLE recovery ranged from 90 to 103% for urine samples and solid phase extraction recovery ranged from 80 to 107% for hair samples. Long-Evans rats (n = 55) were administered 25B-, 25C-or 25I-NBOMe at doses ranging from 30 to 300 mu g/kg over a period of 10 days. Rats were shaved prior to their first dose and re-shaved after the 10-day period. Hair was separated by color (black: n = 55 and white: n = 55) and analyzed using the validated LC-MS-MS method to assess the impact hair color has on the incorporation of these drugs. All drugs were successfully detected in black hair. 25B-NBOMe from rats receiving the highest dose and 25C-NBOMe from rats receiving the medium and high doses were quantified in white hair. 25I-NBOMe was detected but fell below the limit of quantification. A dose-dependent concentration increase was observed in the black hair. All pooled urine samples tested positive for their expected NBOMes.
机译：NBOMes是一组从苯乙胺中提取的新型精神活性物质。娱乐性虐待被认为始于2010年，通常与“俱乐部毒品”场景有关。由于其高效力，它们以液体形式或作为吸墨剂施用。使用法医毒理学参数科学工作组对LC-MS-MS方法进行了验证，该方法用于检测25B-、25C-和4-碘-2,5-二甲氧基-N-[（2-甲氧基苯基）甲基]-苯乙胺（25I NBOMe），使用4-溴-2,5-二甲氧基-N-[（2-甲氧基苯基）甲基]-苯乙胺（25B NBOMe）-D-3作为尿液和头发的内标。在浓度范围分别为0.1至100 ng/mL和0.025至2.5 ng/mg的尿液和头发中观察到R-2值>0.99的校准图。尿液LOD范围为5至25 pg/mL，LOQ为50 pg/mL。头发LOD和LOQ分别为3至5 pg/mg和6.25至12.5 pg/mg。两种基质的日内和日间精密度均<20%，准确度均在+/-20%范围内。结果表明，该方法对外源和内源化合物都具有选择性。两种基质均未观察到基质效应。尿液样本的LLE回收率为90%至103%，头发样本的固相萃取回收率为80%至107%。Long Evans大鼠（n=55）在10天的时间内，以30至300μg/kg的剂量服用25B、25C或25I NBOMe。大鼠在第一次给药前剃毛，10天后再剃毛。头发按颜色（黑色：n=55和白色：n=55）分离，并使用经验证的LC-MS-MS方法进行分析，以评估头发颜色对这些药物掺入的影响。在黑发中成功检测到所有药物。在白发中量化接受最高剂量的大鼠的25B NBOMe和接受中、高剂量的大鼠的25C NBOMe。检测到25I NBOMe，但低于定量限值。在黑发中观察到剂量依赖性浓度增加。所有汇集的尿液样本的预期NBOME检测均呈阳性。

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