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首页> 外文期刊>Journal of biochemical and molecular toxicology >Bilobalide assuages morphine-induced addiction in hippocampal neuron cells through upregulation of microRNA-101
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Bilobalide assuages morphine-induced addiction in hippocampal neuron cells through upregulation of microRNA-101

机译：通过MicroRNA-101的上调，白甲醛诱导吗啡诱导的海马神经元细胞成瘾

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摘要

Bilobalide exhibits many biological activities, but its effects on morphine stimulation have not been elucidated. The research aims to explore the function and underlying mechanisms of bilobalide in morphine-led hippocampal neuron cells. Cells were treated with or without morphine or oxaliplatin (OXA), bilobalide, or SCH772984 dilutions. miR-101 inhibitor and negative control were transfected into cells. Western blot and quantitative reverse transcription-polymerase chain reaction were, respectively, conducted to measure the relative expression of proteins or RNAs. Morphine improved the expression levels of orexinl receptor (OX1R) and c-FOS, the p/t-ERK/PKC as well. The c-FOS protein level and p/t-ERK/PKC were significantly elevated by morphine + OXA. Bilobalide had no effect on OX1R and p/t-PKC but evidently decreased the c-FOS and p/t-ERK. The p-ERK and the c-FOS accumulation levels were remarkably reduced by SCH772984. The production of miR-101 was promoted by bilobalide but inhibited by the miR-101 inhibitor. miR-101 inhibitor abolished bilobalide's inhibitory effects on p/t-ERK. Bilobalide exhibited morphine-induced effects on hippocampal neuron cells by upregulating miR-101.
机译：银杏内酯具有多种生物活性，但其对吗啡刺激的作用尚未阐明。本研究旨在探讨银杏内酯在吗啡诱导的海马神经元细胞中的作用及其潜在机制。用或不用吗啡或奥沙利铂（OXA）、银杏内酯或SCH772984稀释液处理细胞。将miR-101抑制剂和阴性对照转染细胞。westernblot和定量逆转录聚合酶链反应分别用于测量蛋白质或RNA的相对表达。吗啡能提高食欲素受体（OX1R）和c-FOS、p/t-ERK/PKC的表达水平。吗啡+OXA可显著提高c-FOS蛋白水平和p/t-ERK/PKC。银杏内酯对OX1R和p/t-PKC无影响，但明显降低c-FOS和p/t-ERK。SCH772984显著降低了p-ERK和c-FOS的积累水平。bilobalide促进了miR-101的产生，但被miR-101抑制剂抑制。miR-101抑制剂消除了银杏内酯对p/t-ERK的抑制作用。银杏内酯通过上调miR-101对海马神经元细胞表现出吗啡诱导的效应。

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