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首页> 外文期刊>Animal Feed Science and Technology >Using microbial fatty acids to improve understanding of the contribution of solid associated bacteria to microbial mass in the rumen
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Using microbial fatty acids to improve understanding of the contribution of solid associated bacteria to microbial mass in the rumen

机译：使用微生物脂肪酸来增进对固体伴生细菌对瘤胃微生物质量的贡献的了解

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This study sought to distinguish liquid-(LAB) and detached (SAB) and undetached (SAB) solid-associated bacteria through their fatty acid (FA) and purine base (PB) profiles. Fatty acids and PB were also evaluated as internal microbial markers for estimating microbial biomass associated with rumen particles. Four merino rams fitted with rumen cannulae and fed dehydrated alfalfa pellets provided rumen contents. In 3 consecutive weeks, rumen contents were collected and samples of LAB and SAB, total rumen content (TRC), washed rumen particles (WRP) and rumen particles after SAB extraction (ERP) were obtained and analysed for PB and FA. The SAB biomass composition was estimated from the non-NDF organic matter (OM) remaining in ERP. The concentration of total SAB biomass in particles was estimated using both PB and odd and branched-chain fatty acids (OBCFA). Concentrations of PB and OBCFA were highly correlated among the different rumen fractions. Marked differences between LAB and SAB populations occurred with LAB having higher PB content, lower FA content and a higher proportion (g/100g fatty acids) of OBCFA than did SAB. The chemical composition of SAB and SAB was similar, except for the 15% higher crude protein content of the latter. The concentration of OBCFA (mg/g microbial OM) did not differ between bacterial fractions. The PB/OBCFA ratio (mg/mg) was higher in LAB (2.08) than in SAB (0.94). The ratio between branched-chain and odd-linear-chain FA was higher in LAB (2.26) than in SAB (1.46). Extraction of PB and OBCFA from WRP with our SAB detachment procedure was 61% and 31%, respectively. Estimated SAB and total SAB biomass (mg OM/g WRP) were 158 and 266, and 47 and 164, respectively, using PB and OBCFA as microbial markers. This study suggests that the OBCFA have potential as internal microbial markers in rumen ecosystem studies.
机译：这项研究试图通过其脂肪酸（FA）和嘌呤碱基（PB）的特征来区分液体（LAB）和分离的（SAB）和未分离的（SAB）固体相关细菌。脂肪酸和PB也被评估为内部微生物标记，用于估计与瘤胃颗粒相关的微生物生物量。装有瘤胃插管和饲喂脱水苜蓿颗粒的四只美利奴绵羊公羊提供了瘤胃含量。连续3周收集瘤胃含量，并获取LAB和SAB样品，总瘤胃含量（TRC），洗涤瘤胃颗粒（WRP）和SAB提取后瘤胃颗粒（ERP）并进行PB和FA分析。 SAB生物量组成是根据ERP中剩余的非NDF有机物（OM）估算的。使用PB以及奇数和支链脂肪酸（OBCFA）估算颗粒中总SAB生物量的浓度。 PB和OBCFA的浓度在不同瘤胃组分之间高度相关。与SAB相比，LAB的PB含量更高，FA含量更低，OBBCA的比例更高（g / 100g脂肪酸）时，LAB和SAB群体之间出现明显差异。 SAB和SAB的化学成分相似，只是后者的粗蛋白含量高15％。细菌级分之间的OBCFA浓度（mg / g微生物OM）没有差异。 LAB（2.08）中的PB / OBCFA比（mg / mg）高于SAB（0.94）。 LAB（2.26）中的支链FA和奇数线性链FA之比高于SAB（1.46）。用我们的SAB分离程序从WRP中提取PB和OBCFA分别为61％和31％。使用PB和OBCFA作为微生物标记，估计的SAB和总SAB生物量（mg OM / g WRP）分别为158和266，以及47和164。这项研究表明，OBCFA在瘤胃生态系统研究中作为内部微生物标志物具有潜力。

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