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首页> 外文期刊>Journal of genetics >Evaluation of Drosophila chromosomal segments proposed by means of simulations of possessing hybrid sterility genes from reproductive isolation
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Evaluation of Drosophila chromosomal segments proposed by means of simulations of possessing hybrid sterility genes from reproductive isolation

机译：通过对生殖分离的杂交不良基因的模拟提出的果蝇染色体段的评价

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摘要

In heterozygote state, we interogressed three chromosomal segments ofDrosophila koepferaeinD. buzzatii. The effect of each introgression was evaluated in the fertility of the segmental males, quantifying the amount of offspring produced. Through specific crosses method, we generatedDrosophilasegmental isolines carrying specific chromosomal introgression segments. The introgressions were monitored cytogenetically by the method of molecular markers of chromosomal asynapsis. The statistical analysis showed that none of the three segments evaluated, introgressed individually or in pairs, as well ascisortrans, do not produce sterility in the segmental males, as determined by the normal productions of offspring. Additional introgressions using other larger segments show that when the introgressions reach a minimum size of 31.15%, they produce sterility. It is concluded that the hybrid sterility genes present in the three segments evaluated did not act in strong epistasis, but show a pattern of gradual additive behaviour by requiring a minimum threshold size to produce sterility. Finally, we also isolated the smallest introgressing segment that has been reported for these species (2.19%), and for the first time we have managed to place it in homozygous state (data not shown), so we are now in the process of evaluating the ability to these segments in homozygous state.
机译：在杂合子状态下，我们将嗜迷迭香的三个染色体片段进行了互聚合。布扎蒂。每一次渐渗对节段雄性生殖能力的影响都进行了评估，量化了产生后代的数量。通过特异性杂交方法，我们产生了携带特异性染色体导入片段的嗜Rosophilase片段等位基因系。采用染色体不稳定分子标记法对基因导入进行细胞遗传学监测。统计分析表明，根据后代的正常生产情况，单独或成对导入的三个片段，以及ascisortrans，都不会在片段雄性中产生不育。使用其他较大片段的额外导入表明，当导入达到最小大小31.15%时，它们会产生不育。可以得出结论，在所评估的三个片段中存在的杂交不育基因并不在强上位性中起作用，而是通过要求最小阈值大小来产生不育性，表现出一种逐渐加性的行为模式。最后，我们还分离出了已知的这些物种最小的渐渗片段（2.19%），并首次将其置于纯合状态（数据未显示），因此我们现在正在评估将这些片段置于纯合状态的能力。

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