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Validation of a short-term methane measurement using portable static chambers to estimate daily methane production in sheep

机译:使用便携式静态气室评估绵羊每日甲烷产量的短期甲烷测量结果的验证

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There is increasing demand for technologies to measure CH4 production (DMP) of ruminant livestock in inventory and mitigation research. Studies of genetic variation among animals in DMP require these emissions to be measured on thousands of animals which cannot be managed using traditional chamber studies. However, short-term emission measures have been reported to correlate well with DMP. Our study was conducted to determine the usefulness of 1 and 2h emission measures using chambers in predicting DMP. In our first experiment, Merino sheep (n =13) were measured for DMP over 22 h using open circuit chambers. On two subsequent days, after overnight fasts, sheep were offered feed for 1 h before return to the chambers for 2 h and their CH4 production determined. DMP estimated from 22 h measurements correlated moderately well with 2 h emission measures (r(2) of 0.42-0.48) and 1 h measures (r(2) of 0.39-0.43). In our second experiment, portable static chambers were designed for 1 and 2 h CH4 emission measurements. The portable static chambers retained 98-99% of an injected tracer gas after 2 h showing that gas leakage from the chamber was very low. With a sheep inside a portable static chamber, CO2 concentration reached 2.2 x 10(3) ppmv after 2 h, but sheep showed no discomfort and the partial pressure of O-2 in their blood remained above 97% (i.e., safe for animal health). Our third experiment was to validate use of short-term emission measures from portable static chambers as predictors of DMP. Crossbred Dorset and Border Leicester and Merino ewes (n = 40, LW 54 +/- 10.9 kg) were measured for three 22 h sessions in open circuit chambers, after which they were measured for 1 h in portable static chambers. Open circuit chamber measurements had high repeatability (i.e., 0.88) and, excluding one sheep with inconsistent eating patterns, the correlation (r) between 1 h portable static chamber measurements and average emissions in the open circuit chambers was 0.71. We conclude that 1 and 2 h measures of CH4 output in portable static chambers are useful for determining genetic differences in CH4 production in groups of ruminants.This article is part of the special issue entitled: Greenhouse Gases in Animal Agriculture Finding a Balance between Food and Emissions, Guest Edited by TA. McAllister, Section Guest Editors; K.A. Beauchemin, X. Hao, S. McGinn and Editor for Animal Feed Science and Technology, PH. Robinson
机译:在清单和缓解研究中,对用于测量反刍家畜CH4产量(DMP)的技术的需求不断增长。 DMP中动物间遗传变异的研究要求对数千种动物进行这些排放的测量,而这些动物无法使用传统的箱室研究进行管理。然而,据报道,短期排放措施与DMP密切相关。我们的研究是为了确定使用1和2h排放量测量法在预测DMP中的有用性。在我们的第一个实验中,使用开路腔室在22小时内测量了美利奴羊(n = 13)的DMP。在随后的两天中,在禁食过夜后,向绵羊提供了1小时的饲料,然后返回到孵化室2小时并确定了它们的CH4产生。从22小时的测量值估算出的DMP与2小时的排放量测量值(r(2)为0.42-0.48)和1小时的测量值(r(2)为0.39-0.43)适度相关。在我们的第二个实验中,便携式静态室设计用于1和2 h CH4排放测量。便携式静态腔室在2小时后保留了注入的示踪气体的98-99%,表明从腔室泄漏的气体非常少。将绵羊放在便携式静态室内时,CO 2浓度在2小时后达到2.2 x 10(3)ppmv,但是绵羊没有不适感,血液中O-2的分压保持在97%以上(即对动物健康安全) )。我们的第三个实验是验证便携式静态腔室中的短期排放量作为DMP的预测指标的使用。在开路试验箱中对杂交多西特和边境莱斯特和美利奴母羊(n = 40,LW 54 +/- 10.9 kg)进行了三个22小时的测量,然后在便携式静态试验箱中对其进行了1小时的测量。开路腔室测量具有较高的可重复性(即0.88),并且排除一只进食方式不一致的绵羊,便携式1小时静态腔室测量与开路腔室中平均排放量之间的相关性(r)为0.71。我们得出结论,便携式静室中CH4输出的1小时和2小时测量对于确定反刍动物组CH4产生的遗传差异很有用。本文是《动物农业中的温室气体在食物与食物之间找到平衡》这一特刊的一部分。排放,来宾,由TA编辑。麦卡利斯特,客座编辑; K A。 Beauchemin,X. Hao,S. McGinn,动物饲料科学与技术编辑,PH。罗宾逊

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