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首页> 外文期刊>Journal of Medical Entomology >Identification of Lice Stored in Alcohol Using MALDI-TOF MS
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Identification of Lice Stored in Alcohol Using MALDI-TOF MS

机译：使用MALDI-TOF MS鉴定储存在酒精中的虱子

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摘要

Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized.The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.
机译：虱子对公众和兽医健康构成重大问题，并带来经济后果。它们的识别至关重要，需要制定创新战略。MALDI-TOF MS最近被认为是一种快速、廉价、准确的节肢动物鉴定工具。酒精是最常用的储存方法之一，使样品能够在室温下长期储存。最近的几项研究表明，酒精会改变质谱分析得出的蛋白质图谱。在对冷冻虱子进行初步研究后，本研究的目的是评估酒精保存对MALDI-TOF MS识别虱子准确性的影响。为此，将在酒精中储存不同时间的虱子提交给MS分析，并优化样品制备方案。在这两个节肢动物科获得的MS光谱的重复性和特异性使我们能够利用储存在酒精中的七种虱子的蛋白质图谱来实现参考MS光谱数据库（DB）。盲法测试显示，93.9%的人体虱（Linnaeus，1758）和98.4%的其他现场采集的虱子物种得到了正确识别。这项研究表明，MALDI-TOF MS可以成功地用于识别储存在酒精中不同时间长度的虱子。

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