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首页> 外文期刊>Journal of Medical Virology >Characterization of the nasopharyngeal viral microbiome from children with community-acquired pneumonia but negative for Luminex xTAG respiratory viral panel assay detection
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Characterization of the nasopharyngeal viral microbiome from children with community-acquired pneumonia but negative for Luminex xTAG respiratory viral panel assay detection

机译:患有社区获得性肺炎的儿童的鼻咽病毒微生物瘤的特征,但对Luminex XTAG呼吸道病毒组测定检测的阴性

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摘要

In the present study, 50 nasopharyngeal swabs from children with community-acquired pneumonia (CAP) but negative for 18 common respiratory viruses, as measured by the Luminex xTAG Respiratory Viral Panel Assay, were subjected to multiplex metagenomic analyses using a next-generation sequencing platform. Taxonomic analysis showed that all sequence reads could be assigned to a specific species. An average of 95.13% were assigned to the Bacteria kingdom, whereas, only 0.72% were potentially virus derived. This snapshot of the respiratory tract virome revealed most viral reads to be respiratory tract related, classified into four known virus families: Paramyxoviridae, Herpesviridae, Anelloviridae, and Polyomaviridae. Importantly, we detected a novel human parainfluenza virus 3 (HPIV 3) strain with a 32-bp insertion in the haemagglutinin-neuraminidase (HN) gene that produced a negative result in the Luminex assay, highlighting the strength of virome metagenomic analysis to identify not only novel viruses but also viruses likely to be missed by ordinary clinical tests. Thus, virome metagenomic analysis could become a viable clinical diagnostic method.
机译:在本研究中,使用下一代测序平台对来自社区获得性肺炎(CAP)儿童但18种常见呼吸道病毒阴性的50个鼻咽拭子进行多重宏基因组分析,这些拭子通过Luminex xTAG呼吸道病毒平板试验进行测定。分类学分析表明,所有序列读取都可以分配给特定的物种。平均有95.13%属于细菌界,而只有0.72%属于潜在的病毒来源。这张呼吸道病毒组快照显示,大多数病毒读数与呼吸道有关,分为四个已知病毒家族:副粘病毒科、疱疹病毒科、无瘤病毒科和多瘤病毒科。重要的是,我们检测到一种在血凝素神经氨酸酶(HN)基因中插入32 bp的新型人类副流感病毒3(HPIV 3)株,该株在Luminex分析中产生了阴性结果,突出了病毒组宏基因组分析的优势,不仅可以识别新病毒,还可以识别普通临床试验可能遗漏的病毒。因此,病毒组宏基因组分析可能成为一种可行的临床诊断方法。

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