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首页> 外文期刊>Journal of Reproductive Immunology >Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface
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Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface

机译：上皮膜蛋白2（EMP2）在母形界面调节先天免疫细胞群募集

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摘要

Epithelial membrane protein 2 (EMP2) is a tetraspan membrane protein that has been revealed in cancer and placental models to mediate a number of vascular responses. Recently, Emp2 modulation has been shown to have an immunologic effect on uterine NK cell recruitment in the mouse placenta. Given the importance of immune cell populations on both placental vascularization and maternal immune tolerance of the developing fetus, we wanted to better characterize the immunologic effects of Emp2 at the placental-fetal interface. We performed flow cytometry of WT and Emp2 KO C57Bl/6 mouse uterine horns at GD12.5 to characterize immune cell populations localized to the various components of the maternal-fetal interface. We found that Emp2 KO decidua and placenta showed an elevated overall percentage of CD45+ cells compared to WT. Characterization of CD45+ cells in the decidua of Emp2 KO dams revealed an increase in NK cells, whereas in the placenta, Emp2 KO dams showed an increased percentage of M1 macrophages (with an increased ratio of M1/M2 macrophages). Given the differences detected in uNK cell populations in the decidua, we further characterized the interaction between Emp2 genetic KO and NK cell deletion via anti-asialo GM1 antibody injections. While the double knock-out of Emp2 and NK cells did not alter individual pup birthweight, it significantly reduced total litter weight and size by similar to 50 %. In conclusion, Emp2 appears to regulate uNK and macrophage cell populations in pregnancy.
机译：上皮膜蛋白2（EMP2）是一种四跨膜蛋白，已在癌症和胎盘模型中发现，可介导多种血管反应。最近，Emp2调节已被证明对小鼠胎盘中的子宫NK细胞募集具有免疫作用。鉴于免疫细胞群对发育中胎儿的胎盘血管化和母体免疫耐受的重要性，我们希望更好地描述Emp2在胎盘-胎儿界面的免疫效应。我们在GD12处对WT和Emp2 KO C57Bl/6小鼠子宫角进行了流式细胞术。5.描述定位于母胎界面不同成分的免疫细胞群。我们发现，Emp2-KO蜕膜和胎盘中的CD45+细胞总体百分比比WT高。Emp2-KO母鼠蜕膜中的CD45+细胞特征显示NK细胞增加，而在胎盘中，Emp2-KO母鼠的M1巨噬细胞百分比增加（M1/M2巨噬细胞比率增加）。鉴于蜕膜中uNK细胞群中检测到的差异，我们通过抗asialo GM1抗体注射进一步描述了Emp2基因KO和NK细胞缺失之间的相互作用。虽然Emp2和NK细胞的双重敲除并没有改变个别幼崽的出生体重，但它显著降低了幼崽的总重量和大小，约为50%。总之，Emp2似乎可以调节妊娠期的uNK和巨噬细胞数量。

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