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首页> 外文期刊>Journal of Parasitology >MOLECULAR CHARACTERIZATION OF HYPODERMA SPP. IN DOMESTIC RUMINANTS FROM TURKEY AND PAKISTAN
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MOLECULAR CHARACTERIZATION OF HYPODERMA SPP. IN DOMESTIC RUMINANTS FROM TURKEY AND PAKISTAN

机译：Hypoderma SPP的分子表征。 来自土耳其和巴基斯坦的国内反刍动物

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摘要

The aim of this study was to determine the morphological and molecular characterization of Hypoderma spp. in cattle and yak from provinces in Turkey and Pakistan. In total, 78 Hypoderma larvae were collected from slaughtered animals in Turkey and Pakistan from October 2015 to January 2016. Thirty-eight of these 78 Hypoderma larvae were morphologically classified as third instar larvae (L3s) of Hypoderma bovis, 37 were classified as Hypoderma lineatum, and 3 were classified as suspected or unidentified. The restriction enzyme TaqI was used to differentiate the Hypoderma spp. by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). According to the sequences and the PCR-RFLP results, all larval samples from cattle from Turkey were classified as H. bovis, except for 1 sample classified as H. lineatum. All Hypoderma larvae from Pakistan were classified as H. lineatum from cattle and as Hypoderma sinense from yak. This study provides the first molecular characterization of H. lineatum (cattle) and H. sinense (yak) in Pakistan based on PCR-RFLP and sequencing results.
机译：本研究的目的是确定土耳其和巴基斯坦各省的牛和牦牛中皮下皮菌的形态和分子特征。2015年10月至2016年1月，土耳其和巴基斯坦共从屠宰动物中采集了78只皮下幼虫。在这78个皮下组织幼虫中，38个在形态学上被归类为牛皮下组织的三龄幼虫（L3s），37个被归类为线性皮下组织，3个被归类为疑似或不明。用限制性内切酶TaqI通过聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）来区分皮下真菌。根据序列和PCR-RFLP结果，来自土耳其的牛的所有幼虫样本均被归类为牛H.bovis，但1个样本被归类为线性H.lineatum。所有来自巴基斯坦的黑皮幼虫都被归类为牛的线状黑皮绦虫和牦牛的黑皮绦虫。本研究基于PCR-RFLP和测序结果，首次对巴基斯坦的线状H.lineatum（牛）和中国H.sinense（牦牛）进行了分子鉴定。

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