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首页> 外文期刊>Annals of thoracic and cardiovascular surgery: official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia >Replacement of dynamic cultured biograft improves damaged heart function-comparative study of static cultured biografts
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Replacement of dynamic cultured biograft improves damaged heart function-comparative study of static cultured biografts

机译：替代动态培养的生物移植可改善受损的心脏功能-静态培养的生物移植的对比研究

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Purpose: To determine whether a dynamic cultured biograft can positively affect the function of the damaged heart. Methods: We ligated the coronary artery (LAD) of rats to generate a model of myocardial infarction (MI) and then implanted them with the following grafts comprising vascular smooth muscle cells (VSMCs) derived from the rat aorta and seeded onto biodegradable patches (patch replacement therapy; (PRTx)): control without PRTx, PRTx without seeded cells, PRTx with static cultured VSMCs, PRTx with dynamic cultured VSMCs and sham-operated. Cultured VSMCs were labeled with PKH26 for identification after implantation, and the centre of the MI site was excised and replaced with an implanted biograft. Cardiac performance was monitored for 12 weeks thereafter and followed by a histological study. Results: Although the ejection fraction of the damaged heart improved in all groups that were transplanted with grafts, remodeling was prevented only in groups with a dynamic or static cultured patch. More cells were α-SMA-positive in the group with the dynamic, rather than the static cultured patch. Cells were positive for PKH26 in the biograft and in the infarcted myocardium. Conclusions: Dynamic cultured biografts improved the function of the infarcted myocardium more than statically cultured biografts or those without cells.
机译：目的：确定动态培养的生物移植物是否可以对受损心脏的功能产生积极影响。方法：我们将大鼠的冠状动脉（LAD）结扎以生成心肌梗塞（MI）模型，然后将其植入以下包含来自大鼠主动脉的血管平滑肌细胞（VSMC）并植入可生物降解的斑块（斑块）的移植物中替代疗法;（PRTx））：无PRTx的对照，无种子细胞的PRTx，带有静态培养的VSMC的PRTx，带有动态培养的VSMC的PRTx和假手术。植入后，用PKH26标记培养的VSMC，以进行鉴定，并切除MI部位的中心，并用植入的生物移植物代替。此后12周监测心脏表现，随后进行组织学研究。结果：尽管在所有移植了移植物的组中受损心脏的射血分数均得到改善，但只有在动态或静态培养斑块的组中才可以防止重塑。具有动态而非静态培养斑块的组中更多的细胞是α-SMA阳性。在生物移植物中和在梗塞的心肌中，细胞对PKH26呈阳性。结论：动态培养的生物移植物比静态培养的生物移植物或无细胞的生物移植物对梗塞心肌的功能改善更大。

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