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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >The effects of cross-linking a collagen-elastin dermal template on scaffold bio-stability and degradation
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The effects of cross-linking a collagen-elastin dermal template on scaffold bio-stability and degradation

机译：交联胶原蛋白 - 弹性蛋白皮肤模板对支架生物稳定性和降解的影响

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摘要

MatriDerm is a collagen-elastin dermal template that promotes regeneration in full-thickness wound repair. Due to its noncross-linked status, MatriDerm biodegrades quickly in a wound. Facilitating vascularization and dermal repair, it is desirable for MatriDerm to remain present until the wound healing process is complete, optimizing tissue regeneration and reducing wound contraction. The aim of this study was to investigate the effect of cross-linking MatriDerm on its mechanical and biological properties and to enhance its regenerative functionality. MatriDerm was chemically cross-linked and characterized in comparison with noncross-linked MatriDerm. Scaffold properties including surface morphology, protein release and mechanical strength were assessed. Cell-scaffold interaction, cell proliferation and migration were examined using human dermal fibroblasts. Scaffold biodegradation and its impact on wound healing and contraction were studied in a mouse model. Results showed that cross-linked MatriDerm displayed a small reduction in pore size, significantly less protein loss and a threefold increase in tensile strength. A significant increase in fibroblast proliferation and migration was observed in cross-linked MatriDerm with reduced scaffold contraction in vitro. In the mouse model, noncross-linked MatriDerm was almost completely biodegraded after 14 days whereas cross-linked MatriDerm remained intact. No significant difference in wound contraction was found between scaffolds. In conclusion, cross-linked MatriDerm showed a significant increase in stability and strength, enhancing its durability and cell-scaffold interaction. in vivo analysis showed cross-linked MatriDerm had a reduced biodegradation rate with a similar host response. The extended structural integrity of cross-linked MatriDerm could potentially facilitate improved skin tissue regeneration, promoting the formation of a more pliable scar.
机译：MatriDerm是一种胶原蛋白弹性蛋白真皮模板，可促进全层伤口修复中的再生。由于其非交叉连接状态，MatriDerm在伤口中会迅速降解。促进血管化和真皮修复，在伤口愈合过程完成之前，MatriDerm需要保持存在，从而优化组织再生并减少伤口收缩。本研究的目的是研究交联基质对其力学和生物学性能的影响，并增强其再生功能。MatriDerm是化学交联的，并与非交联MatriDerm进行了比较。评估了支架的表面形态、蛋白质释放和机械强度等性能。利用人真皮成纤维细胞检测细胞-支架相互作用、细胞增殖和迁移。在小鼠模型中研究了支架生物降解及其对伤口愈合和收缩的影响。结果表明，交联基质的孔径略有减小，蛋白质损失显著减少，拉伸强度增加三倍。在交联基质层中观察到成纤维细胞增殖和迁移显著增加，体外支架收缩减少。在小鼠模型中，14天后，非交联基质层几乎完全生物降解，而交联基质层保持完整。支架之间的伤口收缩没有显著差异。综上所述，交联基质材料的稳定性和强度显著提高，增强了其耐久性和细胞-支架相互作用。体内分析表明，交联基质的生物降解率降低，宿主反应相似。交联基质层的扩展结构完整性可能有助于改善皮肤组织再生，促进更柔韧疤痕的形成。

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