摘要：Artificial dermis today lacks vascular network and is slow in angiogenesis in vivo. Controlled delivery of angiogenin(ANG)，a potent inducer of angiogenesis，would promote angiogenesis of the artificial dermis. In present study，a collagen/chitosan porous scaffold was fabricated and heparinized by freeze-drying method. Using radioiodinelabeling method the effect of heparin on the binding of ANG to the scaffold，as well as the 16 days’ release of ANG from the scaffold，was investigated. In vivo angiogenesis and degradation of the scaffold was studied for a period of 28 days. With a mean pore size of 96.0 μm,heparinized scaffold possessed three-dimensional porous structure morphologies. Binding of ANG to the scaffold showed a linear correlation with the ANG concentrations. Up to concentrations of 160 ng /ml,binding of ANG to heparinized scaffold was 36.5％. In vitro，ANG was released from the heparinized scaffold in a controlled manner. The controlled release of ANG enhanced angiogenesis of the heparinized scaffold after implantation subcutaneously in rabbits，and the angiogenesis accelerates the degradation of the scaffold. The results of this study indicate that collagen/chitosan porous scaffold laded with ANG may be valuable for the development of the artificial dermis requiring enhanced angiogenesis.
