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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Laser therapy increases the proliferation of preosteoblastic MC3T3-E1 cells cultured on poly(lactic acid) films
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Laser therapy increases the proliferation of preosteoblastic MC3T3-E1 cells cultured on poly(lactic acid) films

机译：激光治疗增加了在聚（乳酸）薄膜上培养的预煮粥MC3-E1细胞的增殖

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摘要

This study aimed to verify the efficacy of low-level laser irradiation (LLLI) on the proliferation of MC3T3-E1 preosteoblasts cultured on poly(lactic acid) (PLA) films. The produced films were characterized by contact angle tests, scanning electron microscopy (SEM), atomic force microscopy, differential scanning calorimetry, and X-ray diffraction. The MC3T3-E1 cells were cultured as three different groups: Control-cultured on polystyrene plastic surfaces; PLA-cultured on PLA films; and PLA + Laser-cultured on PLA films and submitted to laser irradiation (660 nm; 30 mW; 4 J/cm(2)). Cell proliferation was analyzed by Trypan blue and Alamar blue assays at 24, 48, and 72 h after irradiation. Cell viability was assessed by Live/Dead assay, apoptosis-related events were evaluated by Annexin V/propidium iodide (PI) expression, and cell cycle events were analyzed by flow cytometry. Cell morphology on the surface of films was assessed by SEM. Cell counting and biochemical assay results indicate that the PLA + Laser group exhibited higher proliferation (p< 0.01) when compared with the Control and PLA groups. The Live/Dead and Annexin/PI assays indicate increased cell viability in the PLA + Laser group that also presented a higher percentage of cells in the proliferative cell cycle phases (S and G2/M). These findings were also confirmed by the higher cell density observed in the irradiated group through SEM images. The evidence from this study supports the idea that LLLI increases the proliferation of MC3T3-E1 cells on PLA surfaces, suggesting that it can be potentially applied in bone tissue engineering.
机译：本研究旨在验证低强度激光照射（LLLI）对在聚乳酸（PLA）膜上培养的MC3T3-E1前成骨细胞增殖的影响。通过接触角测试、扫描电子显微镜（SEM）、原子力显微镜、差示扫描量热法和X射线衍射对制备的薄膜进行了表征。MC3T3-E1细胞培养分为三组：对照组在聚苯乙烯塑料表面培养；在解放军电影中培养解放军；PLA+激光在PLA膜上培养并接受激光照射（660nm；30mW；4J/cm（2））。在照射后24、48和72小时，通过台盼蓝和阿拉玛蓝分析细胞增殖。通过活/死实验评估细胞活力，通过膜联蛋白V/碘化丙啶（PI）表达评估凋亡相关事件，并通过流式细胞术分析细胞周期事件。用扫描电镜观察了薄膜表面的细胞形态。细胞计数和生化分析结果表明，与对照组和PLA组相比，PLA+激光组表现出更高的增殖（p<0.01）。活/死和膜联蛋白/PI分析表明，PLA+激光组的细胞活力增加，在增殖细胞周期阶段（S和G2/M）细胞比例也较高。通过扫描电镜图像观察到受照组细胞密度较高，这也证实了这些发现。这项研究的证据支持LLLI增加PLA表面MC3T3-E1细胞增殖的观点，表明它可能在骨组织工程中应用。

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