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首页> 外文期刊>Acta Physiologiae Plantarum >Effects of genotype, light regime, explant position and orientation on direct somatic embryogenesis from leaf explants of Phalaenopsis orchids
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Effects of genotype, light regime, explant position and orientation on direct somatic embryogenesis from leaf explants of Phalaenopsis orchids

机译：基因型，光照，外植体位置和方向对蝴蝶兰叶片外植体直接体细胞胚发生的影响

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摘要

The influence of light regime, explant position and orientation on direct embryo formation from leaf explants of two Phalaenopsis, P. amabilis and P. Nebula, were investigated to optimize the protocol for regenerating of this orchid. When explants were cultured in light, direct embryogenesis was retarded in both species. Embryos showed whitish to pale green in color and larger size than those cultured in darkness. Furthermore, light regime induced explant browning, embryo necrosis and eventually low plantlet conversion rate. Sixty days of culture in darkness is the most suitable duration for direct embryo induction. Explant orientation also significantly affected direct embryo formation, and explants placed adaxial-side-up on culture medium had higher embryogenic response than abaxial-side-up orientation. In both species, the cut end had highest embryogenic competence than other parts of the explant. Moreover, when the leaf explant was cut transversely into two segments, the leaf basal segment had higher embryogenic competence than the leaf tip segment.
机译：研究了光照方式，外植体位置和方向对两个蝴蝶兰（P. amabilis和P. Nebula）叶片外植体直接形成胚的影响，​​以优化该兰花的再生方案。当在光下培养外植体时，两个物种的直接胚胎发生均受到阻碍。胚胎的颜色为白色至浅绿色，比在黑暗中培养的颜色更大。此外，轻度诱导外植体褐变，胚坏死并最终导致低的植株转化率。在黑暗中培养60天是直接诱导胚胎的最合适时间。外植体的取向也显着影响直接胚胎的形成，并且在培养液中近侧朝上放置的外植体比远侧朝上放置的胚发生反应更高。在这两个物种中，切端比外植体的其他部分具有最高的胚发生能力。此外，当将叶片外植体横向切成两段时，叶基段比叶尖段具有更高的胚发生能力。

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