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首页> 外文期刊>Acta Physiologiae Plantarum >Loss-of-function of DELLA protein SLN1 activates GA signaling in barley aleurone
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Loss-of-function of DELLA protein SLN1 activates GA signaling in barley aleurone

机译：DELLA蛋白SLN1的功能丧失激活大麦糊粉糊中的GA信号传导

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摘要

Gibberellic acid (GA) is an important signaling molecule that participates in many aspects of plant growth and development. While the importance of this hormone is clear, the transcriptional regulatory networks involved are still being characterized. The cereal aleurone, particularly the barley aleurone, has been used as a classic model to study GA and GA signaling for many years, and these studies have significantly contributed to our understanding of GA in plant biology. The objective of this study was to characterize the transcripts regulated through the DELLA protein SLN1, a negative regulator of the GA signaling pathway. To detect the transcripts, Affymetrix Barley 1 GeneChips were hybridized with RNA extracted from barley aleurone treated with GA or aleurone of the DELLA mutant sln1c without GA treatment. The transcripts detected, in term of both expressed genes and their function, were highly similar between the GA-treatment and the sln1c mutant. These results from a genome-wide transcript analysis provide evidence that SLN1 in the GA signal transduction pathway controls almost all GA-induced genes in the barley aleurone.
机译：赤霉素（GA）是一种重要的信号分子，参与植物生长和发育的许多方面。尽管这种激素的重要性很明确，但所涉及的转录调控网络仍在表征中。谷物糊粉，尤其是大麦糊粉被用作研究GA和GA信号转导的经典模型已有很多年了，这些研究为我们对植物生物学中GA的理解做出了重要贡献。这项研究的目的是表征通过DELLA蛋白SLN1（GA信号通路的负调节剂）调节的转录物。为了检测转录本，将Affymetrix大麦1基因芯片与从用GA处理的大麦糊粉或未经GA处理的DELLA突变体sln1c的糊粉中提取的RNA杂交。检测到的转录本，无论是表达的基因还是它们的功能，在GA处理和sln1c突变体之间都非常相似。来自全基因组转录本分析的这些结果提供了证据，表明GA信号转导途径中的SLN1控制着大麦糊粉中几乎所有GA诱导的基因。

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