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首页> 外文期刊>Annals of Agricultural Science >In Vitro Morpho-histological Studies of New Developed Embryos From Abnormal Malformed Embryos of Date Palm cv. Gundila Under Desiccation Effect of Polyethelyne Glycol Treatments
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In Vitro Morpho-histological Studies of New Developed Embryos From Abnormal Malformed Embryos of Date Palm cv. Gundila Under Desiccation Effect of Polyethelyne Glycol Treatments

机译：从枣棕榈变态畸形胚胎中新发育的胚胎的体外形态学研究。甘地拉在聚乙炔乙二醇处理的干燥作用下

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摘要

Substantial progress has been made towards micropropagation of date palm species by somatic embryogenesis. Routine production faced disadvantage due to persisting problems with the efficiency of embryo maturation and conversion. This was attributed toformation of somatic embryos with numerous morphological abnormalities. The present paper was conducted to study the desiccation effect of poly ethylene glycol (PEG) in relation to embryo maturation, and germination. Abnormal malformed embryos explantswere cultured on germination medium supplemented with different concentrations of PEG (15, 20 and 25 gl ) for two period (15, 30 days) as desiccations treatments. Clear significant changes showed de novo organs as new direct somatic embryos (secondary embryos). These embryos could be germinated with shoots formation from all desiccated abnormal somatic embryos explants with PEG treatments. The addition of PEG at 20 gl1' to germination medium for 30 days gave the highest significant number of new directsomatic embryos comparing with other treatments. Finally secondary somatic embryos converted to full plantlets. For the first time histological investigation was made to reveal anatomical development of secondary embryos from abnormal malformed embryos under (PEG) treatments. The first step showed numerous meristematic multicellular centers occurred before treatment with (PEG).These centers may be serve as inactive source of embryoids. Some morphological and histological changes were recorded during (PEG) treatments and determined by special developmental stages (2-celled, 4-celled, globular and bipolar embryos with normal structure) of new somatic embryos of date palm. The presented data strongly supported that the osmotic stress caused by different concentrations of PEG activate the process of morpho-ontogenetic events to develop new healthy somatic embryos.
机译：通过体细胞胚发生对椰枣种进行微繁已经取得了实质性进展。由于持续存在的胚胎成熟和转化效率问题，常规生产面临劣势。这归因于具有许多形态异常的体细胞胚的形成。本文旨在研究聚乙二醇（PEG）的脱水作用与胚胎成熟和萌发的关系。将异常畸形的胚胎外植体在补充有不同浓度PEG（15、20和25 gl）的发芽培养基上培养两个时期（15、30天），进行干燥处理。明显的显着变化表明新生器官为新的直接体细胞胚（次生胚）。这些胚胎可以通过PEG处理从所有干燥的异常体细胞胚外植体中形成芽并萌发。与其他处理方法相比，在20 gl1'处将PEG加入发芽培养基中30天，可以产生最高数量的新的直接体细胞胚。最后，次生体细胞胚转化为完整的幼苗。首次进行组织学研究以揭示在（PEG）处理下来自异常畸形胚胎的次生胚胎的解剖发育。第一步显示在用（PEG）治疗之前发生了许多分生性多细胞中心，这些中心可能是胚状体的非活性来源。在（PEG）处理期间记录了一些形态学和组织学变化，这些变化由枣核的新体细胞胚的特殊发育阶段（具有正常结构的2细胞，4细胞，球状和双极胚胎）确定。提出的数据强烈支持由不同浓度的PEG引起的渗透胁迫激活形态发生事件的过程，以发展出新的健康的体细胞胚。

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