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首页> 外文期刊>Applied Microbiology and Biotechnology >Batch and fed-batch bioreactor cultivations of a Thermus species with thermophilic BTEX-degrading activity
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Batch and fed-batch bioreactor cultivations of a Thermus species with thermophilic BTEX-degrading activity

机译：具有嗜热BTEX降解活性的栖热菌种的分批和补料分批生物反应器培养

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摘要

The thermophilic bacterium, Thermus species ATCC 27978, which is capable of aerobically degrading benzene, toluene, ethylbenzene, and the xylenes (BTEX), was cultured in 5-1 fermenters on a Castenholz salts-tryptone medium. This bacterium can be cultivated more conveniently at 45 degrees C, a temperature substantially lower than its optimal growth temperature (approx. 60 degrees C). Yet, the washed harvested cells from such cultures display the same initial BTEX-degrading activity as those when Thermus sp. is grown at its higher optimal temperature. Two bioreactor cultivation modes, batch and fed batch, were investigated. More biomass and more BTEX-degrading activity (assayed at 60 degrees C) were generated in fed-batch cultures than in the growth-limited batch cultures. The former yielded a biomass concentration of 2.5 g dry cell weight (DCW) 1(-1) and whole-cell degrading specific activities of 7.6 +/- 1.3, 10.1 +/- 1.9, 9.8 +/- 2.1 2.3 +/- 0.5, and 4.6 +/- 0.9 nmol degraded (mg DCW)(-1) min(-1) for benzene, toluene, ethylbenzene, m-xylene, and the o- plus p-xylenes (unresolved mixture), respectively. Although the formation of cellular BTEX-degrading activity is growth-associated, a slow to moderate specific growth rate of 0.02 - 0.07 h(-1) favors the production of BTEX-degrading activity, while a high growth rate, of the order of 0.16 h(-1), is detrimental to its production. The washed harvested Thermus sp. cells were capable of degrading BTEX over a broad range of thermophilic incubation temperatures, 45-77 degrees C.
机译：能够在需氧条件下降解苯，甲苯，乙苯和二甲苯（BTEX）的嗜热菌Thermus种ATCC 27978在Castenholz盐-培养基上的5-1发酵罐中培养。该细菌可以在45摄氏度（温度大大低于其最佳生长温度（约60摄氏度））下更方便地培养。然而，从这种培养物中洗涤得到的收获的细胞显示出与Thermus sp。时相同的初始BTEX降解活性。在更高的最佳温度下生长。研究了两种生物反应器培养模式，分批和补料分批。分批补料培养比限制生长的分批培养产生更多的生物量和更多的BTEX降解活性（在60摄氏度下测定）。前者产生的生物量浓度为2.5 g干细胞重量（DCW）1（-1），全细胞降解的比活为7.6 +/- 1.3、10.1 +/- 1.9、9.8 +/- 2.1 2.3 +/- 0.5和4.6 +/- 0.9 nmol分别对苯，甲苯，乙苯，间二甲苯和邻-对对二甲苯（未分解的混合物）降解（mg DCW）（-1）min（-1）。尽管细胞BTEX降解活性的形成与生长相关，但是0.02至0.07 h（-1）的缓慢至中等的比生长速率有利于BTEX降解活性的产生，而高生长速率约为0.16 h（-1），不利于其产生。洗涤后的收获的Thermus sp.。细胞能够在45-77摄氏度的广泛嗜热温育温度范围内降解BTEX。

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