...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >Batch, design optimization, and DNA sequencing study for continuous 1,3-propanediol production from waste glycerol by a soil-based inoculum
【24h】

Batch, design optimization, and DNA sequencing study for continuous 1,3-propanediol production from waste glycerol by a soil-based inoculum

机译：基于土壤接种物的废甘油连续生产1,3-丙二醇的批处理，设计优化和DNA测序研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

1,3-propanediol (1,3-PD) was produced with a robust fermentation process using waste glycerol feedstock from biodiesel production and a soil-based bacterial inoculum. An iterative inoculation method was developed to achieve independence from soil and selectively breed bacterial populations capable of glycerol metabolism to 1,3-PD. The inoculum showed high resistance to impurities in the feedstock. 1,3-PD selectivity and yield in batch fermentations was optimized by appropriate nutrient compositions and pH control. The batch yield of 1,3-PD was maximized to -0.7 mol/mol for industrial glycerol which was higher than that for pure glycerin. 16S rDNA sequencing results show a systematic selective enrichment of 1,3-PD producing bacteria with iterative inoculation and subsequent process control. A statistical design of experiments was carried out on industrial glycerol batches to optimize conditions, which were used to run two continuous flow stirred-tank reactor (CSTR) experiments over a period of > 500 h each. A detailed analysis of steady states at three dilution rates is presented. Enhanced specific 1,3-PD productivity was observed with faster dilution rates due to lower levels of solvent degeneration. 1,3-PD productivity, specific productivity, and yield of 1.1 g/l hr, 1.5 g/g hr, and 0.6 mol/mol of glycerol were obtained at a dilution rate of 0.1 h(-1)which is bettered only by pure strains in pure glycerin feeds.
机译：1,3-丙二醇（1,3-PD）使用生物柴油生产的废甘油原料和基于土壤的细菌接种物，通过强大的发酵过程生产。开发了一种迭代接种方法，以实现与土壤的独立性，并选择性繁殖能够将甘油代谢为1,3-PD的细菌种群。接种物显示出对原料中杂质的高抗性。通过适当的营养成分和pH控制，可以优化分批发酵中1,3-PD的选择性和产量。工业甘油的1，3-PD的批量产率最大为-0.7mol / mol，高于纯甘油。 16S rDNA测序结果表明，通过反复接种和随后的过程控制，系统地选择性富集了1,3-PD产生细菌。对工业甘油批料进行了统计设计的实验，以优化条件，该条件用于运行两个连续流搅拌釜反应器（CSTR）实验，每次> 500小时。给出了三种稀释率下稳态的详细分析。由于较低的溶剂变性水平，在较快的稀释速率下观察到了更高的1,3-PD比生产率。 1,3-PD的生产率，比生产率和1.1 g / l hr，1.5 g / g hr的产率以及0.6 mol / mol甘油的稀释率为0.1 h（-1），这只能通过纯甘油饲料中的纯菌株。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号