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首页> 外文期刊>Applied Microbiology and Biotechnology >Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach
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Direct quantification of fatty acids in wet microalgal and yeast biomass via a rapid in situ fatty acid methyl ester derivatization approach

机译：通过快速原位脂肪酸甲酯衍生化方法直接定量测定湿微藻和酵母生物量中的脂肪酸

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摘要

Accurate determination of fatty acid contents is routinely required in microalgal and yeast biofuel studies. A method of rapid in situ fatty acid methyl ester (FAME) derivatization directly from wet fresh microalgal and yeast biomass was developed in this study. This method does not require prior solvent extraction or dehydration. FAMEs were prepared with a sequential alkaline hydrolysis (15 min at 85 A degrees C) and acidic esterification (15 min at 85 A degrees C) process. The resulting FAMEs were extracted into n-hexane and analyzed using gas chromatography. The effects of each processing parameter (temperature, reaction time, and water content) upon the lipids quantification in the alkaline hydrolysis step were evaluated with a full factorial design. This method could tolerate water content up to 20 % (v/v) in total reaction volume, which equaled up to 1.2 mL of water in biomass slurry (with 0.05-25 mg of fatty acid). There were no significant differences in FAME quantification (p > 0.05) between the standard AOAC 991.39 method and the proposed wet in situ FAME preparation method. This fatty acid quantification method is applicable to fresh wet biomass of a wide range of microalgae and yeast species.
机译：通常在微藻和酵母生物燃料研究中需要准确测定脂肪酸含量。本研究开发了一种直接从湿的新鲜微藻和酵母生物质中直接衍生的快速脂肪酸甲酯（FAME）的方法。该方法不需要事先进行溶剂萃取或脱水。通过连续的碱水解（在85 A摄氏度下15分钟）和酸性酯化（在85 A摄氏度下15分钟）过程制备FAME。将所得的FAME萃取到正己烷中，并使用气相色谱法进行分析。使用全因子设计评估了每个加工参数（温度，反应时间和水含量）对碱性水解步骤中脂质定量的影响。该方法可耐受总反应体积中高达20％（v / v）的水含量，相当于生物质浆料中的水含量高达1.2 mL（含0.05-25 mg脂肪酸）。在标准AOAC 991.39方法与拟议的湿法原位FAME制备方法之间，FAME定量没有显着差异（p> 0.05）。这种脂肪酸定量方法适用于各种微藻和酵母种类的新鲜湿生物质。

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