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首页> 外文期刊>Applied Microbiology and Biotechnology >Fermentation of biodiesel-derived glycerol by Bacillus amyloliquefaciens: effects of co-substrates on 2,3-butanediol production
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Fermentation of biodiesel-derived glycerol by Bacillus amyloliquefaciens: effects of co-substrates on 2,3-butanediol production

机译:淀粉芽孢杆菌发酵生物柴油衍生的甘油:共底物对2,3-丁二醇生产的影响

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摘要

Cultivation in glycerol instead of sugars inhibits 2,3- butanediol (2,3-BD) production by Bacillus amyloliquefaciens. In this study, we report that B. amyloliquefaciens readily produces 2,3-BD from biodiesel-derived glycerol in the presence of beet molasses as a co-substrate. Unexpectedly, the molasses stimulated 2,3-BD production and simultaneously reduced the duration of fermentation. Productivity of 2,3-BD was enhanced at the start of fermentation, and yields increased under continuous molasses supply. Subsequently, 2,3-BD production in molasses-supplemented fed-batch culture was observed. Prior to inoculation of fed-batch fermentation culture, 15 g/l of molasses was added to the bioreactor. After 6 h of incubation, the bioreactor was fed with a solution containing 80 % glycerol and 15 % molasses. The 2,3-BD concentration, yield, and productivity significantly improved, reaching 83.3 g/l, 0.42 g/g, and 0.87 g/l.h, respectively. To our knowledge, these results are the highest report for 2,3-BD fermentation from biodieselderived glycerol.
机译:在甘油而不是糖中的培养抑制了解淀粉芽孢杆菌的2,3-丁二醇(2,3-BD)产生。在这项研究中,我们报道在甜菜糖蜜作为共同底物的存在下,解淀粉芽孢杆菌很容易从生物柴油衍生的甘油中产生2,3-BD。出乎意料的是,糖蜜刺激了2,3-BD的产生并同时减少了发酵的持续时间。在发酵开始时,2,3-BD的生产率提高,并且在连续糖蜜供应下产量增加。随后,观察到在糖蜜补充的分批补料培养物中产生了2,3-BD。在接种分批补料发酵培养物之前,将15 g / l的糖蜜添加到生物反应器中。温育6小时后,向生物反应器中加入含有80%甘油和15%糖蜜的溶液。 2,3-BD的浓度,产量和生产率均得到显着提高,分别达到83.3 g / l,0.42 g / g和0.87 g / l.h。据我们所知,这些结果是由生物柴油衍生的甘油进行的2,3-BD发酵的最高报告。

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