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首页> 外文期刊>Applied Microbiology and Biotechnology >γ-Heptalactone is an endogenously produced quorum-sensing molecule regulating growth and secondary metabolite production by Aspergillus nidulans
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γ-Heptalactone is an endogenously produced quorum-sensing molecule regulating growth and secondary metabolite production by Aspergillus nidulans

机译：γ-庚内酯是内生的群体感应分子，可调节构巢曲霉的生长和次级代谢产物

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摘要

Microbes monitor their population density through a mechanism termed quorum sensing. It is believed that quorum-sensing molecules diffuse from the microbial cells and circulate in the surrounding environment as a function of cell density. When these molecules reach a threshold concentration, the gene expression of the entire population is altered in a coordinated manner. This work provides evidence that Aspergillus nidulans produces at least one small diffusible molecule during its growth cycle which accumulates at high cell density and alters the organism's behaviour. When added to low-density cell cultures, ethyl acetate extracts from stationary phase culture super-natants of A. nidulans resulted in the abolition of the lag phase, induced an earlier deceleration phase with 16.3 % decrease in the final cell dry weight and resulted in a 37.8 % increase in the expression of ipnA:lacZ reporter gene construct, which was used as a marker for penicillin production compared to non-treated controls. The bioactive molecule present in the stationary phase extract was purified to homogeneity and was identified by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy to be γ-heptalactone. This study provides the first evidence that A. nidulans produces γ-heptalactone at a high cell density and it can alter the organism's behaviour at a low cell density, γ-Heptalactone hence acts as a quorum-sensing molecule in the producing strain.
机译：微生物通过称为群体感应的机制监控种群密度。据信群体感应分子从微生物细胞扩散并在周围环境中循环，这是细胞密度的函数。当这些分子达到阈值浓度时，整个种群的基因表达将以协调的方式改变。这项工作提供了证据，构巢曲霉在其生长周期中产生至少一个小的可扩散分子，该分子以高细胞密度积累并改变了生物体的行为。当添加到低密度细胞培养物中时，构巢曲霉固定相培养上清液的乙酸乙酯提取物可消除滞后相，诱导较早的减速相，最终细胞干重减少16.3％，从而导致ipnA：lacZ报告基因构建体的表达增加了37.8％，与未处理的对照相比，ipnA：lacZ报告基因构建体被用作青霉素生产的标记。固定相提取物中存在的生物活性分子被纯化至均质，并通过液相色谱-质谱和核磁共振波谱鉴定为γ-庚内酯。这项研究提供了第一个证据，即构巢曲霉以高细胞密度产生γ-庚内酯，并且可以在低细胞密度下改变生物体的行为，因此，γ-庚内酯在产生菌株中起群体感应分子的作用。

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