Saccharide production from methanol by transposon 5 mutants derived from the extracellular polysaccharide-producing bacterium Methylobacillus sp strain 12S

Yoshida T.; Ayabe Y.; Yamaguchi T.; Shibuya N.; Habe H. Nojiri H.; Yamane H.; Omori T.; Horinouchi M.

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Saccharide production from methanol by transposon 5 mutants derived from the extracellular polysaccharide-producing bacterium Methylobacillus sp strain 12S

机译：通过转座子5突变体从甲醇中生产糖精，这些突变体来自产生细胞外多糖的细菌甲基杆菌sp菌株12S

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A CH3OH-utilizing bacterium that has the ability to produce extracellular polysaccharide (EPS) was isolated from a soil sample, and was identified as the obligate methylotroph Methylobacillus sp. strain 12S on the basis of its 16S. rDNA sequence and growth-substrate specificity. The EPS produced by strain 12S was purified and the sugar composition was analysed by CC-MS and HPLC to reveal that the EPS was a heteropolymer composed of glucosyl, galactosyl, and mannosyl residues in the molar ratio 3:1:1. In order to produce mono- and/or oligosaccharides by single-step fermentation from CH3OH, stain 12S was mutagenized by transposon 5. Among eleven EPS-deficient mutants, three strains were found to accumulate significant amounts of reducing sugars in the media. The amounts of the reducing sugars produced by the mutants (> ca. 700 mg glucose equivalent/l) were >11-22 times higher than those produced by the wild-type strain (

机译：从土壤样品中分离出具有CH3OH利用能力的细菌，该细菌具有产生细胞外多糖（EPS）的能力，并被确定为专性甲基营养型甲基杆菌属。菌株12S在其16S的基础上。 rDNA序列和生长底物特异性。纯化了由菌株12S产生的EPS，并通过CC-MS和HPLC分析了糖的组成，发现EPS是由葡糖基，半乳糖基和甘露糖基残基组成的杂聚物，摩尔比为3：1：1。为了通过单步发酵从CH3OH产生单糖和/或寡糖，用转座子5诱变染色剂12S。在11个EPS缺陷型突变体中，发现有3个菌株在培养基中积累了大量还原糖。突变体产生的还原糖量（>约700毫克葡萄糖当量/升）比野生型菌株产生的还原糖（<约60毫克葡萄糖当量/升）高11-22倍。 CC-MS分析表明，所有突变体均在培养基中积累了葡萄糖，赤藓糖，苏糖和类似二糖的化合物。 [参考：33]

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《Applied Microbiology and Biotechnology》 |2000年第3期|共7页
作者
Yoshida T.; Ayabe Y.; Yamaguchi T.; Shibuya N.; Habe H. Nojiri H.; Yamane H.; Omori T.; Horinouchi M.;
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正文语种 eng
中图分类微生物学;
关键词
C1 assimilation; Biosynthesis; Mobilization; Alignment; Enzymes;

机译：C1同化;生物合成;动员;比对;酶;

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1. Saccharide production from methanol by transposon 5 mutants derived from the extracellular polysaccharide-producing bacterium Methylobacillus sp strain 12S [J] . Yoshida T., Ayabe Y., Yamaguchi T., Applied Microbiology and Biotechnology . 2000,第3期

机译：通过转座子5突变体从甲醇中生产糖精，这些突变体来自产生细胞外多糖的细菌甲基杆菌sp菌株12S
2. Description of five mutants of the cyanobacterium Anabaena sp strain PCC 7120 affected in heterocyst differentiation and identification of the transposon-tagged genes [J] . Maldener I., Hannus S., Kammerer M. FEMS Microbiology Letters . 2003,第2期

机译：蓝藻鱼腥藻PCC 7120的五个突变体在异型囊肿分化中的影响以及转座子标记基因的鉴定
3. Genes involved in the synthesis of the exopolysaccharide methanolan by the obligate methylotroph Methylobacillus sp. strain 12S [J] . Takako Yoshida, Yuko Ayabe, Masaaki Yasunaga, Microbiology . 2003,第2期

机译：通过迫使甲基双甲基甲基溴磺酸铋合成外荷甲醇的基因。菌株12s.
4. An Expression of a Cold Shock Inducible Gene CspA Under Hydrostatic Pressure in Deep-Sea Barophilic Bacterium, Shewanella sp. Strain DSS12 [C] . S. Fujii, K. Nakasone, K. Horikoshi International conference on high pressure bioscience and biotechnology . 1999

机译：深海嗜酸体菌静压压力下冷却诱导基因CSPA的表达，雪兰菜SP。菌株DSS12
5. The synthesis and characterization of novel biomaterials from natural building blocks: Biomaterials research: Past, present, and future directions, and, Saccharide-derived polymers as protein resistant biomaterials, and, Saccharide-peptide copolymers for gene delivery applications, and, Saccharide-peptide copolymer hydrogels for extracellular scaffold design. [D] . Metzke, Mark Adam. 2006

机译：从天然构件中合成和表征新型生物材料：生物材料研究：过去，现在和未来的方向，以及，糖衍生的聚合物作为抗蛋白质生物材料，以及用于基因传递应用的糖肽共聚物，以及糖肽共聚物水凝胶用于细胞外支架设计。
6. The Chlorocatechol-Catabolic Transposon Tn5707 of Alcaligenes eutrophus NH9 Carrying a Gene Cluster Highly Homologous to That in the 124-Trichlorobenzene-Degrading Bacterium Pseudomonas sp. Strain P51 Confers the Ability To Grow on 3-Chlorobenzoate [O] . Naoto Ogawa, Kiyotaka Miyashita 1999

机译：拟南芥NH9的氯邻苯二酚-转座子Tn5707携带与在124-三氯苯降解细菌假单胞菌中高度同源的基因簇。 P51菌株具有3-氯苯甲酸酯的生长能力
7. Construction of Hfr-like donors of the obligate methanol-oxidizing bacterium Methylobacillus flagellatum KT [O] . I.G. Serebrijski, S.M. Kazakova, Y.D. Tsygankov 1989

机译：Chillage甲醇氧化菌甲基溴虫蛋白氟氯虫KT的HFR样供体的构建

Saccharide production from methanol by transposon 5 mutants derived from the extracellular polysaccharide-producing bacterium Methylobacillus sp strain 12S

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