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首页> 外文期刊>Applied Microbiology and Biotechnology >The two putative comS homologs of the biotechnologically important Bacillus licheniformis do not contribute to competence development
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The two putative comS homologs of the biotechnologically important Bacillus licheniformis do not contribute to competence development

机译：生物技术上重要的地衣芽孢杆菌的两个推定的comS同源物不会促进能力发展

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摘要

In Bacillus subtilis, natural genetic competence is subject to complex genetic regulation and quorum sensing dependent. Upon extracellular accumulation of the peptide-pheromone ComX, the membrane-bound sensor histidine kinase ComP initiates diverse signaling pathways by activating-among others-DegQ and ComS. While DegQ favors the expression of extracellular enzymes rather than competence development, ComS is crucial for competence development as it prevents proteolytic degradation of ComK, the key transcriptional activator of all genes required for the uptake and integration of DNA. In Bacillus licheniformis, ComX/ComP sensed cell density negatively influences competence development, suggesting differences from the quorum-sensing-dependent control mechanism in Bacillus subtilis. Here, we show that each of six investigated strains possesses both of two different, recently identified putative comS genes. When expressed from an inducible promoter, none of the comS candidate genes displayed an impact on competence development neither in B. subtilis nor in B. licheniformis. Moreover, disruption of the genes did not reduce transformation efficiency. While the putative comS homologs do not contribute to competence development, we provide evidence that the degQ gene as for B. subtilis negatively influences genetic competency in B. licheniformis.
机译：在枯草芽孢杆菌中，自然遗传能力受复杂的遗传调控和群体感应的影响。在肽-信息素ComX的细胞外积累后，膜结合的传感器组氨酸激酶ComP通过激活其他分子（DegQ和ComS）来启动多种信号通路。尽管DegQ促进细胞外酶的表达而非能力发展，但ComS对于能力发展至关重要，因为它阻止了ComK的蛋白水解降解，ComK是摄取和整合DNA所需的所有基因的关键转录激活因子。在地衣芽孢杆菌中，ComX / ComP感知到的细胞密度对能力发展产生负面影响，表明与枯草芽孢杆菌中群体感应依赖性控制机制的差异。在这里，我们显示六个调查菌株中的每一个都拥有两个不同的，最近确定的推定的comS基因。当从诱导型启动子表达时，没有一个comS候选基因在枯草芽孢杆菌和地衣芽孢杆菌中都没有显示出对能力发展的影响。而且，基因的破坏并没有降低转化效率。虽然推定的comS同源物不会促进能力发展，但我们提供的证据表明，枯草芽孢杆菌的degQ基因会对地衣芽孢杆菌的遗传能力产生负面影响。

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