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首页> 外文期刊>Applied Microbiology and Biotechnology >Methylotrophy in the thermophilic Bacillus methanolicus, basic insights and application for commodity production from methanol
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Methylotrophy in the thermophilic Bacillus methanolicus, basic insights and application for commodity production from methanol

机译：嗜热芽孢杆菌中的甲基营养，甲醇的商品生产的基本见解和应用

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摘要

Using methanol as an alternative non-food feedstock for biotechnological production offers several advantages in line with a methanol-based bioeconomy. The Gram-positive, facultative methylotrophic and thermophilic bacterium Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value-added products. Its capabilities of producing and secreting the commercially important amino acids l-glutamate and l-lysine to high concentrations at 50 A degrees C have been demonstrated and make B. methanolicus a promising target to develop cell factories for industrial-scale production processes. B. methanolicus uses the ribulose monophosphate cycle for methanol assimilation and represents the first example of plasmid-dependent methylotrophy. Recent genome sequencing of two physiologically different wild-type B. methanolicus strains, MGA3 and PB1, accompanied with transcriptome and proteome analyses has generated fundamental new insight into the metabolism of the species. In addition, multiple key enzymes representing methylotrophic and biosynthetic pathways have been biochemically characterized. All this, together with establishment of improved tools for gene expression, has opened opportunities for systems-level metabolic engineering of B. methanolicus. Here, we summarize the current status of its metabolism and biochemistry, available genetic tools, and its potential use in respect to overproduction of amino acids.
机译：与基于甲醇的生物经济相一致，使用甲醇作为生物技术生产的替代非食品原料具有许多优势。革兰氏阳性，兼性甲基营养和嗜热细菌甲醇芽孢杆菌是描述的少数几种可能将甲醇转化为增值产品的微生物候选物之一。已经证明了其在50 A的温度下能够生产和分泌高浓度的商业上重要的氨基酸L-谷氨酸和L-赖氨酸的能力，并使甲醇芽孢杆菌成为发展用于工业规模生产工艺的细胞工厂的有希望的目标。甲醇芽孢杆菌使用核糖一磷酸循环进行甲醇同化，并代表了质粒依赖性甲基营养的第一个例子。最近对两种生理上不同的野生型甲醇毕赤酵母菌株MGA3和PB1进行基因组测序，并进行了转录组和蛋白质组分析，从而对该物种的代谢产生了基础性的新见解。另外，已经表征了代表甲基营养和生物合成途径的多种关键酶。所有这些，以及建立改进的基因表达工具，为甲醇双歧杆菌的系统级代谢工程打开了机会。在这里，我们总结了其代谢和生物化学的当前状态，可用的遗传工具以及其在氨基酸过量生产方面的潜在用途。

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