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Electrochemical immunosensors for Salmonella detection in food

机译：用于食品中沙门氏菌检测的电化学免疫传感器

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摘要

Pathogen detection is a critical point for the identification and the prevention of problems related to food safety. Failures at detecting contaminations in food may cause outbreaks with drastic consequences to public health. In spite of the real need for obtaining analytical results in the shortest time possible, conventional methods may take several days to produce a diagnosis. Salmonella spp. is the major cause of foodborne diseases worldwide and its absence is a requirement of the health authorities. Biosensors are bioelectronic devices, comprising bioreceptor molecules and transducer elements, able to detect analytes (chemical and/or biological species) rapidly and quantitatively. Electrochemical immunosensors use antibody molecules as bioreceptors and an electrochemical transducer. These devices have been widely used for pathogen detection at low cost. There are four main techniques for electrochemical immunosensors: amperometric, impedimetric, conductometric, and potentiometric. Almost all types of immunosensors are applicable to Salmonella detection. This article reviews the developments and the applications of electrochemical immunosensors for Salmonella detection, particularly the advantages of each specific technique. Immunosensors serve as exciting alternatives to conventional methods, allowing "real-time" and multiple analyses that are essential characteristics for pathogen detection and much desired in health and safety control in the food industry.
机译：病原体检测是识别和预防与食品安全有关的问题的关键点。未能检测到食物中的污染物可能会导致疾病暴发，并对公共卫生造成严重后果。尽管真正需要在尽可能短的时间内获得分析结果，但常规方法可能需要几天才能做出诊断。沙门氏菌是世界范围内食源性疾病的主要原因，而卫生当局的要求是不存在食源性疾病。生物传感器是生物电子设备，包括生物受体分子和换能器元件，能够快速，定量地检测分析物（化学和/或生物种类）。电化学免疫传感器使用抗体分子作为生物受体和电化学传感器。这些设备已经以低成本广泛地用于病原体检测。电化学免疫传感器有四种主要技术：电流法，阻抗法，电导法和电位法。几乎所有类型的免疫传感器都适用于沙门氏菌检测。本文回顾了用于沙门氏菌检测的电化学免疫传感器的发展和应用，特别是每种特定技术的优势。免疫传感器是传统方法的令人兴奋的替代方法，可以进行“实时”和多种分析，这对于病原体检测是必不可少的特征，在食品工业的健康和安全控制中非常需要。

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