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首页> 外文期刊>Applied Microbiology and Biotechnology >Enrichment strategies for nitrile-hydrolysing bacteria
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Enrichment strategies for nitrile-hydrolysing bacteria

机译：腈水解细菌的富集策略

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A series of enrichments with different nitriles as sole source of nitrogen was performed in order to obtain a relationship between the selective nitrogen source and (i) the enzyme systems that are synthesized by the isolates and (ii) the enzyme specificities for the utilization of the nitriles. Bacteria were enriched with 2-phenylpropionitrile, 2-(2-methoxyphenyl)propionitrile 2-phenylbutyronitrile, ibuprofen nitrile, naproxen nitrile, ketoprofen nitrile, ketoprofen amide, benzonitrile. or naphthalenecarbonitrile as sole nitrogen source and succinate as sole source of carbon and energy. 2-Phenylpropionitrile as nitrogen source resulted predominantly in the enrichment of gram-negative bacteria, which harboured nitrilase and in some cases also amidase activity. In contrast, with the other nitriles used, a substantial majority of gram-positive strains, mainly Of the genus Rhodococcus, were isolated. These strains contained predominantly a nitrile hydratase/amidase system. The nitrilases and nitrile hydratases showed R or S selectivity with generally poor optical yields. III contrast, the amidases were almost exclusively S-selective, often forming the optically pure acids with an enantiomeric excess above 99%. The conversion of different nitriles by the isolates was compared. The nitrile-hydrolysing systems of the new isolates usually showed high activity against those nitriles that were used for the enrichment of the bacteria.
机译：为了获得选择性氮源与（i）由分离物合成的酶系统和（ii）利用该酶的酶特异性之间的关系，进行了一系列以不同腈为唯一氮源的富集。腈。细菌富含2-苯基丙腈，2-（2-甲氧基苯基）丙腈2-苯基丁腈，布洛芬腈，萘普生腈，酮洛芬腈，酮洛芬酰胺，苄腈。或萘甲腈作为唯一的氮源，而琥珀酸盐作为唯一的碳和能量源。 2-苯基丙腈作为氮源主要导致革兰氏阴性细菌的富集，该细菌具有腈水解酶，在某些情况下还具有酰胺酶活性。相反，与其他腈一起使用时，分离出了绝大多数革兰氏阳性菌株，主要是红球菌属。这些菌株主要包含腈水合酶/酰胺酶系统。腈水解酶和腈水合酶显示出R或S选择性，且光学收率通常较差。与之相反，酰胺酶几乎完全是S选择性的，通常形成对映体过量超过99％的光学纯酸。比较了分离物对不同腈的转化。新分离株的腈水解系统通常对那些用于细菌富集的腈具有很高的活性。

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