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首页> 外文期刊>Applied Microbiology and Biotechnology >DasR is a pleiotropic regulator required for antibiotic production, pigment biosynthesis, and morphological development in Saccharopolyspora erythraea
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DasR is a pleiotropic regulator required for antibiotic production, pigment biosynthesis, and morphological development in Saccharopolyspora erythraea

机译：DasR是一种多效性调节剂，在糖多孢红霉菌中产生抗生素，色素生物合成和形态发育均需要

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摘要

The GntR-family transcription regulator, DasR, was previously identified as pleiotropic, controlling the primary amino sugar N-acetylglucosamine (GlcNAc) and chitin metabolism in Saccharopolyspora erythraea and Streptomyces coelicolor. Due to the remarkable regulatory impact of DasR on antibiotic production and development in the model strain of S. coelicolor, we here identified and characterized the role of DasR to secondary metabolite production and morphological development in industrial erythromycin-producing S. erythraea. The physiological studies have shown that a constructed deletion of dasR in S. erythraea resulted in antibiotic, pigment, and aerial hyphae production deficit in a nutrient-rich condition. DNA microarray assay, combined with quantitative real-time reverse transcription PCR (qRT-PCR), confirmed these results by showing the downregulation of the genes relating to secondary metabolite production in the dasR null mutant. Notably, electrophoretic mobility shift assays (EMSA) showed DasR as being the first identified regulator that directly regulates the pigment biosynthesis rpp gene cluster. In addition, further studies indicated that GlcNAc, the major nutrient signal of DasR-responsed regulation, blocked secondary metabolite production and morphological development. The effects of GlcNAc were shown to be caused by DasR mediation. These findings demonstrated that DasR is an important pleiotropic regulator for both secondary metabolism and morphological development in S. erythraea, providing new insights for the genetic engineering of S. erythraea with increased erythromycin production.
机译：GntR家族转录调节剂DasR先前被鉴定为多效性，可控制红糖酵母和腔链链霉菌中的主要氨基糖N-乙酰氨基葡萄糖（GlcNAc）和几丁质代谢。由于DasR对天蓝色链霉菌模型菌株中抗生素产生和发展的显着调控作用，我们在此鉴定并表征了DasR在工业红霉素生产中的S. erythraea中对次生代谢产物产生和形态发育的作用。生理学研究表明，在营养丰富的条件下，红藻中dasR的缺失会导致抗生素，色素和气生菌丝的产生。 DNA微阵列测定法与定量实时逆转录PCR（qRT-PCR）结合，通过显示与dasR null突变体中与次级代谢产物产生有关​​的基因的下调，证实了这些结果。值得注意的是，电泳迁移率变动分析（EMSA）显示DasR是第一个直接调节颜料生物合成rpp基因簇的已鉴定调节剂。此外，进一步的研究表明，DasR响应调控的主要营养信号GlcNAc阻止了次级代谢产物的产生和形态发育。已显示GlcNAc的作用是由DasR介导的。这些发现表明，DasR是红细菌中次生代谢和形态发育的重要多效性调节剂，为红霉素生产增加的红细菌基因工程提供了新的见解。

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