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Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process

机译:补料分批过程中的维生素补料策略可提高酿酒酵母的乙醇产量和生存能力

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Several bottlenecks in the alcoholic fermentation process must be overcome to reach a very high and competitive performance of bioethanol production by the yeast Saccharomyces cerevisiae. In this paper, a nutritional strategy is described that allowed S. cerevisiae to produce a final ethanol titre of 19% (v/v) ethanol in 45 h in a fed-batch culture at 30degreesC. This performance was achieved by implementing exponential feeding of vitamins throughout the fermentation process. In comparison to an initial addition of a vitamin cocktail, an increase in the amount of vitamins and an exponential vitamin feeding strategy improved the final ethanol titre from 126 g l(-1) to 135 g l(-1) and 147 g l(-1), respectively. A maximum instantaneous productivity of 9.5 g l(-1) h(-1) was reached in the best fermentation. These performances resulted from improvements in growth, the specific ethanol production rate, and the concentration of viable cells in response to the nutritional strategy. [References: 33]
机译:必须克服酒精发酵过程中的几个瓶颈,以通过酿酒酵母获得非常高的竞争性生物乙醇生产性能。在本文中,描述了一种营养策略,该策略允许酿酒酵母在30℃分批分批培养的45小时内产生最终乙醇滴度为19%(v / v)的乙醇。通过在整个发酵过程中进行维生素的指数补料来实现此性能。与最初添加维生素混合物相比,维生素含量的增加和指数化的维生素喂养策略将最终乙醇滴定度从126 gl(-1)提高到135 gl(-1)和147 gl(-1) , 分别。在最佳发酵中,最大瞬时生产率达到9.5 g l(-1)h(-1)。这些性能归因于生长,特定乙醇生产速率以及对营养策略的反应中活细胞浓度的改善。 [参考:33]

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