...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >Characterization of membrane-bound dehydrogenases from Gluconobacter oxydans 621H via whole-cell activity assays using multideletion strains
【24h】

Characterization of membrane-bound dehydrogenases from Gluconobacter oxydans 621H via whole-cell activity assays using multideletion strains

机译：使用多删除菌株通过全细胞活性测定表征氧化葡糖杆菌621H的膜结合脱氢酶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gluconobacter oxydans, like all acetic acid bacteria, has several membrane-bound dehydrogenases, which oxidize a multitude of alcohols and polyols in a stereo- and regio-selective manner. Many membrane-bound dehydrogenases have been purified from various acetic acid bacteria, but in most cases without reporting associated sequence information. We constructed clean deletions of all membrane-bound dehydrogenases in G. oxydans 621H and investigated the resulting changes in carbon utilization and physiology of the organism during growth on fructose, mannitol, and glucose. Furthermore, we studied the substrate oxidation spectra of a set of strains where the membrane-bound dehydrogenases were consecutively deleted using a newly developed whole-cell 2,6- dichlorophenolindophenol (DCPIP) activity assay in microtiter plates. This allowed a detailed and comprehensive in vivo characterization of each membrane-bound dehydrogenase in terms of substrate specificity. The assays revealed that general rules can be established for some of the enzymes and extended the known substrate spectra of some enzymes. It was also possible to assign proteins whose purification and characterization had been reported previously, to their corresponding genes. Our data demonstrate that there are less membrane-bound dehydrogenases in G. oxydans 621H than expected and that the deletion of all of them is not lethal for the organism.
机译：与所有乙酸细菌一样，氧化葡糖杆菌具有几种膜结合的脱氢酶，它们以立体和区域选择性的方式氧化多种醇和多元醇。已经从各种乙酸细菌中纯化了许多膜结合的脱氢酶，但是在大多数情况下，没有报告相关的序列信息。我们构建了G. oxydans 621H中所有膜结合的脱氢酶的干净缺失，并研究了在果糖，甘露醇和葡萄糖生长过程中生物体内碳利用和生理变化的结果。此外，我们研究了一组菌株的底物氧化光谱，其中使用新开发的全细胞2,6-二氯苯酚吲哚酚（DCPIP）活性测定法在微量滴定板中连续删除了膜结合的脱氢酶。这样就可以根据底物特异性对每种膜结合的脱氢酶进行详细而全面的体内表征。分析表明，可以为某些酶建立通用规则，并扩展了某些酶的已知底物光谱。也可以将其纯化和特征已被报道的蛋白质分配给它们相应的基因。我们的数据表明，氧化G. oxydans 621H中的膜结合脱氢酶比预期的要少，并且所有这些酶的缺失都不会对生物造成致命的伤害。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号