• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >Dynamics of metabolically active bacterial communities involved in PAH and toxicity elimination from oil-contaminated sludge during anoxic/oxic oscillations
【24h】

Dynamics of metabolically active bacterial communities involved in PAH and toxicity elimination from oil-contaminated sludge during anoxic/oxic oscillations

机译：缺氧/含氧振荡过程中参与多环芳烃代谢活动的细菌群落的动态和消除油污污泥的毒性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The kinetics of polycyclic aromatic hydrocarbons (PAH) elimination from a contaminated sludge were determined in bioreactors under different conditions: continuously oxic, anoxic, and anoxic/oxic oscillations. The dynamics of metabolically active bacterial communities and their involvement in PAH degradation were followed by T-RFLP targeting 16S rRNA and ring hydroxylating dioxygenase (RHD) transcripts, respectively. PAH degradation was related to toxicity elimination using an aryl hydrocarbon receptorresponsive reporter cell line. Oxygen supply was identified as the main factor affecting the structure of bacterial communities and PAH removal. PAH-degrading bacterial communities were stable throughout the experiment in all conditions according to the presence of RHD transcripts, indicating that bacterial communities were well adapted to the presence of pollutants. Oxic and anoxic/oxic oscillating conditions showed similar levels of PAH removal at the end of the experiment despite several anoxic periods in oscillating conditions. These results highlight the role of dioxygenase activity after oxygen addition. Nevertheless, the higher toxicity elimination observed under oxic conditions suggests that some metabolites or other unidentified active compounds persisted under oscillating and anoxic conditions. Our results emphasize the importance of using complementary biological, chemical and toxicological approaches to implement efficient bioremediation strategies.
机译：在生物反应器中，在以下不同条件下确定了从污染污泥中去除多环芳烃（PAH）的动力学：连续的有氧，无氧和无氧/有氧振荡。代谢活性细菌群落的动力学及其参与PAH降解的过程分别是靶向16S rRNA和环羟基化双加氧酶（RHD）转录物的T-RFLP。 PAH降解与使用芳烃受体响应性报告细胞系消除毒性有关。氧气供应被认为是影响细菌群落结构和去除PAH的主要因素。根据RHD转录本的存在，在所有条件下，降解PAH的细菌群落在整个实验过程中都是稳定的，这表明细菌群落非常适合污染物的存在。尽管在振荡条件下有几个缺氧时间，但在有氧和缺氧/有氧振荡条件下，实验结束时PAH的去除水平相似。这些结果突出了加氧后双加氧酶活性的作用。然而，在有氧条件下观察到的更高的毒性消除表明某些代谢物或其他未确定的活性化合物在振荡和缺氧条件下仍然存在。我们的结果强调了使用互补的生物学，化学和毒理学方法来实施有效的生物修复策略的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号