...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >The inhibition of aggregation of recombinant human consensus interferon-alpha mutant during Pichia pastoris fermentation
【24h】

The inhibition of aggregation of recombinant human consensus interferon-alpha mutant during Pichia pastoris fermentation

机译：重组人共有干扰素-α突变体在毕赤酵母发酵过程中的聚集抑制

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lower induction temperature and polyoxyethylene sorbitan monolaurate (Tween-20) were successfully used to inhibit the aggregation of recombinant human consensus interferon-alpha mutant (cIFN) during Pichia pastoris fermentation. When the induction temperature was decreased from 30 to 20 degrees C, the cIFN secreted into the medium was in the form of monomers instead of aggregates. The maximum specific activity at 20 degrees C was 4.04 times as high as that at 30 degrees C. There was no obvious effect on the cell growth at 20 degrees C, but the total protein level was decreased. Similar inhibition effect on cIFN aggregation was observed when 0.2 g l(-1) Tween-20 was added during induction. Furthermore, there was a synergistic effect found between induction temperature and Tween-20 on the inhibition of cIFN aggregation. The maximum specific activity with Tween-20 at 20 degrees C was 19.9-fold higher than that without Tween-20 at 30 degrees C.
机译：较低的诱导温度和聚氧乙烯脱水山梨糖醇单月桂酸酯（Tween-20）已成功用于抑制巴斯德毕赤酵母发酵过程中重组人共有干扰素-α突变体（cIFN）的聚集。当诱导温度从30摄氏度降低到20摄氏度时，分泌到培养基中的cIFN呈单体形式而不是聚集体形式。 20℃时的最大比活度是30℃时的4.04倍。20℃时对细胞的生长没有明显影响，但总蛋白水平却降低了。当诱导过程中添加0.2 g l（-1）Tween-20时，观察到对cIFN聚集的类似抑制作用。此外，在诱导温度和Tween-20之间存在抑制cIFN聚集的协同效应。在20摄氏度下使用Tween-20的最大比活度是在30摄氏度下不使用Tween-20的最大比活度的19.9倍。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号