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首页> 外文期刊>Applied Microbiology and Biotechnology >Metabolic response of Bacillus sphaericus 1593M for dual-substrate limitation in continuous and total-cell-retention cultures
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Metabolic response of Bacillus sphaericus 1593M for dual-substrate limitation in continuous and total-cell-retention cultures

机译：球形芽孢杆菌1593M对连续和全细胞保留培养物中双底物限制的代谢反应

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摘要

A chemically defined medium has been developed to support the growth and the production of mosquito larvicidal factor(s) (MLF) of Bacillus sphaericus 1593M. On the basis of the data of steady-state continuous cultures, it has been understood that acetate can serve as a sole carbon and energy source for B. sphaericus 1593M. Utilization of acetate by B. sphaericus 1593M and the production of MLF are further enhanced by the addition of glutamate at low concentrations, both in steady-state continuous as well as in total-cell-retention cultures (TCRC). A two-step TCRC procedure resulted in better biomass and MLF production by B. sphaericus 1593M. It was also found that glutamate can serve as a carbon source as well as a growth factor in the presence of acetate and hence is a partially substitutable carbon source.
机译：已开发出一种化学成分确定的培养基来支持球形芽孢杆菌1593M的蚊虫幼虫因子（MLF）的生长和生产。根据稳态连续培养的数据，已经知道乙酸盐可以作为球形芽孢杆菌1593M的唯一碳和能源。通过在稳态连续培养以及全细胞保留培养（TCRC）中添加低浓度的谷氨酸盐，可进一步提高球形芽孢杆菌1593M对乙酸的利用和MLF的产生。两步TCRC程序导致球形芽孢杆菌1593M产生更好的生物量和MLF。还发现谷氨酸盐可以在乙酸盐存在下充当碳源以及生长因子，因此是部分可取代的碳源。

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